ArticleThe Journal of physiology2004
Sequential activation of RhoA and FAK/paxillin leads to ATP release and actin reorganization in human endothelium.
Article in The Journal of physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 66 citations in OpenAlex.
- Catalase-like activity of CoMikrochimica acta · 2026Article
- An optogenetic model reveals cell shape regulation through FAK and fascin.Journal of cell science · 2021Article
- P2Y Purinergic Receptors, Endothelial Dysfunction, and Cardiovascular Diseases.International journal of molecular sciences · 2020Review
- Network Pharmacology Reveals the Mechanism of Activity of Tongqiao Huoxue Decoction Extract Against Middle Cerebral Artery Occlusion-Induced Cerebral Ischemia-Reperfusion Injury.Frontiers in pharmacology · 2020Article
- Paracrine signalling of AGR2 stimulates RhoA function in fibroblasts and modulates cell elongation and migration.Cell adhesion & migration · 2019Article
- Altering integrin engagement regulates membrane localization of KJournal of cell science · 2019Article
- ROS-dependent activation of RhoA/Rho-kinase in pulmonary artery: Role of Src-family kinases and ARHGEF1.Free radical biology & medicine · 2017Article
- Paxillin: a crossroad in pathological cell migration.Journal of hematology & oncology · 2017Review
- Shear-Sensitive Genes in Aortic Valve Endothelium.Antioxidants & redox signaling · 2016Review
- Mechanosensitive Molecular Networks Involved in Transducing Resistance Exercise-Signals into Muscle Protein Accretion.Frontiers in physiology · 2016Review
- Fyn Mediates High Glucose-Induced Actin Cytoskeleton Reorganization of Podocytes via Promoting ROCK Activation In Vitro.Journal of diabetes research · 2016Article
- A bibenzyl from Dendrobium ellipsophyllum inhibits migration in lung cancer cells.Journal of natural medicines · 2015Article
- Hypoxia is an effective stimulus for vesicular release of ATP from human umbilical vein endothelial cells.Placenta · 2015Article
- Lysophosphatidic acid in atherosclerotic diseases.British journal of pharmacology · 2012Review
- New insights regarding the regulation of chemotaxis by nucleotides, adenosine, and their receptors.Purinergic signalling · 2012Review
- Lysophosphatidic acid receptor 1 modulates lipopolysaccharide-induced inflammation in alveolar epithelial cells and murine lungs.American journal of physiology. Lung cellular and molecular physiology · 2011Article
- Rho signaling regulates pannexin 1-mediated ATP release from airway epithelia.The Journal of biological chemistry · 2011Article
- Nucleotide receptors as targets in the pharmacological enhancement of dermal wound healing.Purinergic signalling · 2011Article
- P2Y1 and P2Y13 purinergic receptors mediate Ca2+ signaling and proliferative responses in pulmonary artery vasa vasorum endothelial cells.American journal of physiology. Cell physiology · 2011Article
- The effects of the small GTPase RhoA on the muscarinic contraction of airway smooth muscle result from its role in regulating actin polymerization.American journal of physiology. Cell physiology · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have investigated the cellular mechanisms of mechanical stress-induced immediate responses in human umbilical vein endothelial cells (HUVECs). Hypotonic stress (HTS) induced ATP release, which evoked a Ca(2+) transient, followed by actin reorganization within a few minutes, in HUVECs. Disruption of the actin cytoskeleton did not suppress HTS-induced ATP release, and inhibition of the ATP-mediated Ca(2+) response did not affect actin reorganization, thereby indicating that these two responses are not interrelated. ATP release and actin reorganization were also induced by lysophosphatidic acid (LPA). HTS and LPA induced membrane translocation of RhoA, which occurs when RhoA is activated, and tyrosine phosphorylation of focal adhesion kinase (FAK) and paxillin. Tyrosine kinase inhibitors (herbimycin A or tyrphostin 46) inhibited both HTS- and LPA-induced ATP release and actin reorganization, but did not affect RhoA activation. In contrast, Rho-kinase inhibitor (Y27632) inhibited all of the HTS- and LPA-induced responses. These results indicate that the activation of the RhoA/Rho-kinase pathway followed by tyrosine phosphorylation of FAK and paxillin leads to ATP release and actin reorganization in HUVECs. Furthermore, the fact that HTS and LPA evoke exactly the same intracellular signals and responses suggests that even these immediate mechanosensitive responses are in fact not mechanical stress-specific.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.