ArticleInfection and immunity2004
Characterization of the icmH and icmF genes required for Legionella pneumophila intracellular growth, genes that are present in many bacteria associated with eukaryotic cells.
Article in Infection and immunity, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 54 citations in OpenAlex.
- Comparative Transcriptomics and Genomics from Continuous Axenic Media Growth IdentifiesbioRxiv : the preprint server for biology · 2023Article
- Genetic Diversity of Antimicrobial Resistance and Key Virulence Features in Two Extensively Drug-ResistantInternational journal of environmental research and public health · 2022Article
- Recent advances in structural studies of the Legionella pneumophila Dot/Icm type IV secretion system.Microbiology and immunology · 2022Review
- Diverse Aquatic Animal Matrices Play a Key Role in Survival and Potential Virulence of Non-O1/O139Frontiers in microbiology · 2022Article
- Role of the type VI secretion systems during disease interactions of Erwinia amylovora with its plant host.BMC genomics · 2017Article
- Article
- Essential role for the response regulator PmrA in Coxiella burnetii type 4B secretion and colonization of mammalian host cells.Journal of bacteriology · 2014Article
- DotU expression is highly induced during in vivo infection and responsible for virulence and Hcp1 secretion in avian pathogenic Escherichia coli.Frontiers in microbiology · 2014Article
- Identification of a functional type VI secretion system in Campylobacter jejuni conferring capsule polysaccharide sensitive cytotoxicity.PLoS pathogens · 2013Article
- The C-tail anchored TssL subunit, an essential protein of the enteroaggregative Escherichia coli Sci-1 Type VI secretion system, is inserted by YidC.MicrobiologyOpen · 2012Article
- DotU and VgrG, core components of type VI secretion systems, are essential for Francisella LVS pathogenicity.PloS one · 2012Article
- Type IVB Secretion Systems of Legionella and Other Gram-Negative Bacteria.Frontiers in microbiology · 2011Article
- Comparative genomic analysis uncovers 3 novel loci encoding type six secretion systems differentially distributed in Salmonella serotypes.BMC genomics · 2009Article
- An IcmF family protein, ImpLM, is an integral inner membrane protein interacting with ImpKL, and its walker a motif is required for type VI secretion system-mediated Hcp secretion in Agrobacterium tumefaciens.Journal of bacteriology · 2009Article
- Identification and functional characterization of gene components of Type VI Secretion system in bacterial genomes.PloS one · 2008Article
- Attenuated Francisella novicida transposon mutants protect mice against wild-type challenge.Infection and immunity · 2006Article
- Role of FNR and FNR-regulated, sugar fermentation genes in Neisseria meningitidis infection.Molecular microbiology · 2006Article
- Additive effect on intracellular growth by Legionella pneumophila Icm/Dot proteins containing a lipobox motif.Infection and immunity · 2005Article
- Exposure to rumen protozoa leads to enhancement of pathogenicity of and invasion by multiple-antibiotic-resistant Salmonella enterica bearing SGI1.Infection and immunity · 2005Article
- sciS, an icmF homolog in Salmonella enterica serovar Typhimurium, limits intracellular replication and decreases virulence.Infection and immunity · 2005Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Legionella pneumophila, the causative agent of Legionnaires' disease, replicates intracellularly within a specialized phagosome of mammalian and protozoan host cells, and the Icm/Dot type IV secretion system has been shown to be essential for this process. Unlike all the other known Icm/Dot proteins, the IcmF protein, which was described before, and the IcmH protein, which is characterized here, have homologous proteins in many bacteria (such as Yersinia pestis, Salmonella enterica, Rhizobium leguminosarum, and Vibrio cholerae), all of which associate with eukaryotic cells. Here, we have characterized the L. pneumophila icmH and icmF genes and found that both genes are present in 16 different Legionella species examined. The icmH and icmF genes were found to be absolutely required for intracellular multiplication in Acanthamoeba castellanii and partially required for intracellular growth in HL-60-derived human macrophages, for immediate cytotoxicity, and for salt sensitivity. Mutagenesis of the predicted ATP/GTP binding site of IcmF revealed that the site is partially required for intracellular growth in A. castellanii. Analysis of the regulatory region of the icmH and icmF genes, which were found to be cotranscribed, revealed that it contains at least two regulatory elements. In addition, an icmH::lacZ fusion was shown to be activated during stationary phase in a LetA- and RelA-dependent manner. Our results indicate that although the icmH and icmF genes probably have a different evolutionary origin than the rest of the icm/dot genes, they are part of the icm/dot system and are required for L. pneumophila pathogenesis.
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