ArticleInfection and immunity2004
Cryptococcus neoformans capsule structure evolution in vitro and during murine infection.
Article in Infection and immunity, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
48 citing papers in PubMed, 74 citations in OpenAlex.
- Synthetic Glycans Reveal Determinants of Antibody Functional Efficacy against a Fungal Pathogen.ACS infectious diseases · 2024Article
- Importance of Clinical Isolates inJournal of fungi (Basel, Switzerland) · 2023Review
- Influence of Pathogen Carbon Metabolism on Interactions With Host Immunity.Frontiers in cellular and infection microbiology · 2022Review
- Article
- The interplay of phenotype and genotype in Cryptococcus neoformans disease.Bioscience reports · 2020Review
- Transposon mobilization in the human fungal pathogenProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Review
- A Glucuronoxylomannan Epitope Exhibits Serotype-Specific Accessibility and Redistributes towards the Capsule Surface during Titanization of the Fungal Pathogen Cryptococcus neoformans.Infection and immunity · 2019Article
- Unraveling synthesis of the cryptococcal cell wall and capsule.Glycobiology · 2018Review
- Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans.Journal of visualized experiments : JoVE · 2018Article
- Cryptococcal pathogenic mechanisms: a dangerous trip from the environment to the brain.Memorias do Instituto Oswaldo Cruz · 2018Review
- First in Vivo Batrachochytrium dendrobatidis Transcriptomes Reveal Mechanisms of Host Exploitation, Host-Specific Gene Expression, and Expressed Genotype Shifts.G3 (Bethesda, Md.) · 2017Article
- Cryptococcus neoformans UGT1 encodes a UDP-Galactose/UDP-GalNAc transporter.Glycobiology · 2017Article
- Polysaccharide Capsule Composition of Pneumococcal Serotype 19A Subtypes Is Unaltered among Subtypes and Independent of the Nutritional Environment.Infection and immunity · 2016Article
- A Small Protein Associated with Fungal Energy Metabolism Affects the Virulence of Cryptococcus neoformans in Mammals.PLoS pathogens · 2016Article
- Aging as an emergent factor that contributes to phenotypic variation in Cryptococcus neoformans.Fungal genetics and biology : FG & B · 2015Review
- Article
- Neuroinvasive cryptococcosis in an immunocompetent patient with a negative spinal fluid cryptococcus antigen.Case reports in infectious diseases · 2015Article
- Aging: an emergent phenotypic trait that contributes to the virulence of Cryptococcus neoformans.Future microbiology · 2015Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryptococcus neoformans capsule structure modifications after prolonged in vitro growth or in vivo passaging have been reported previously. However, nothing is known about the dynamics of these modifications or about their environmental specificities. In this study, capsule structure modifications after mouse passaging and prolonged in vitro culturing were analyzed by flow cytometry using the glucuronoxylomannan-specific monoclonal antibody E1. The capsule structures of strains recovered after 0, 1, 8, and 35 days were compared by using the level of E1-specific epitope expression and its cell-to-cell heterogeneity within a given cell population. In vitro, according to these parameters, the diversity of the strains was higher on day 35 than it was initially, suggesting the absence of selection during in vitro culturing. In contrast, the diversity of the strains recovered from the brain tended to decrease over time, suggesting that selection of more adapted strains had occurred. The strains recovered on day 35 from the spleen and the lungs had different phenotypes than the strains isolated from the brain of the same mouse on the same day, thus strongly suggesting that there is organ specificity for C. neoformans strain selection. Fingerprinting of the strains recovered in vitro and in vivo over time confirmed that genotypes evolved very differently in vitro and in vivo, depending on the environment. Overall, our results suggest that organ-specific selection can occur during cryptococcosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.