ArticleEMBO reports2004
Agrobacterium proteins VirD2 and VirE2 mediate precise integration of synthetic T-DNA complexes in mammalian cells.
Article in EMBO reports, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 45 citations in OpenAlex.
- Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants.International journal of molecular sciences · 2021Review
- Vigilant: An Engineered VirD2-Cas9 Complex for Lateral Flow Assay-Based Detection of SARS-CoV2.Nano letters · 2021Article
- F-Box Gene D5RF Is Regulated by Agrobacterium Virulence Protein VirD5 and Essential for Agrobacterium-Mediated Plant Transformation.International journal of molecular sciences · 2020Article
- An intra-bacterial activity for a T3SS effector.Scientific reports · 2020Article
- Fusion of the Cas9 endonuclease and the VirD2 relaxase facilitates homology-directed repair for precise genome engineering in rice.Communications biology · 2020Article
- Evolvement of nutraceutical onion plants engineered for resveratrol biosynthetic pathway.Plant cell reports · 2019Article
- Transformation by growth onto agro-infiltrated tissues (TGAT), a simple and efficient alternative for transient transformation of the cucurbit powdery mildew pathogen Podosphaera xanthii.Molecular plant pathology · 2018Article
- Agrobacterium-mediated plant transformation: biology and applications.The arabidopsis book · 2017Article
- Transgene expression in tick cells using Agrobacterium tumefaciens.Experimental & applied acarology · 2015Article
- Agrobacterium: nature's genetic engineer.Frontiers in plant science · 2014Article
- Secretory pathway research: the more experimental systems the better.The Plant cell · 2012Review
- A novel method of transgene delivery into triticale plants using the Agrobacterium transferred DNA-derived nano-complex.Plant physiology · 2012Article
- Traversing the Cell: Agrobacterium T-DNA's Journey to the Host Genome.Frontiers in plant science · 2012Article
- Characterization of the single stranded DNA binding protein SsbB encoded in the Gonoccocal Genetic Island.PloS one · 2012Article
- Nuclear targeting of a bacterial integrase that mediates site-specific recombination between bacterial and human target sequences.Applied and environmental microbiology · 2011Article
- VIP1: linking Agrobacterium-mediated transformation to plant immunity?Plant cell reports · 2010Review
- New insights into an old story: Agrobacterium-induced tumour formation in plants by plant transformation.The EMBO journal · 2010Review
- Article
- T-DNA insertion, plasmid rescue and integration analysis in the model mycorrhizal fungus Laccaria bicolor.Microbial biotechnology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Agrobacterium tumefaciens-mediated plant transformation, a unique example of interkingdom gene transfer, has been widely adopted for the generation of transgenic plants. In vitro synthesized transferred DNA (T-DNA) complexes comprising single-stranded DNA and Agrobacterium virulence proteins VirD2 and VirE2, essential for plant transformation, were used to stably transfect HeLa cells. Both proteins positively influenced efficiency and precision of transgene integration by increasing overall transformation rates and by promoting full-length single-copy integration events. These findings demonstrate that the virulence proteins are sufficient for the integration of a T-DNA into a eukaryotic genome in the absence of other bacterial or plant factors. Synthetic T-DNA complexes are therefore unique protein:DNA delivery vectors with potential applications in the field of mammalian transgenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.