ArticleBMC neuroscience2004
Both 5' and 3' flanks regulate Zebrafish brain-derived neurotrophic factor gene expression.
Article in BMC neuroscience, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Knockout in zebrafish suggests a role of BDNF in behavioural and cognitive plasticity.Behavioral and brain functions : BBF · 2026Article
- Long non-coding SINEUP RNA enhancing BDNF translation prevents dendritic atrophy following an Aβ-peptide challenge.Cellular and molecular life sciences : CMLS · 2026Article
- Neurotrophins and their receptors in the peripheral nervous system and non-nervous tissue of fish.Fish physiology and biochemistry · 2025Review
- Loss of circadian rhythmicity iniScience · 2022Article
- Effect of Tempeh on Gut Microbiota and Anti-Stress Activity in Zebrafish.International journal of molecular sciences · 2021Article
- A lysosomal enigma CLN5 and its significance in understanding neuronal ceroid lipofuscinosis.Cellular and molecular life sciences : CMLS · 2021Review
- Learning-Dependent Transcriptional Regulation of BDNF by its Truncated Protein Isoform in Turtle.Journal of molecular neuroscience : MN · 2021Article
- Article
- Neurotrophins in the Brain of Teleost Fish: The State of the Art.Advances in experimental medicine and biology · 2021Review
- BDNF, Brain, and Regeneration: Insights from Zebrafish.International journal of molecular sciences · 2018Review
- Role of brain-derived neurotrophic factor during the regenerative response after traumatic brain injury in adult zebrafish.Neural regeneration research · 2018Review
- Lasting changes induced by mild alcohol exposure during embryonic development in BDNF, NCAM and synaptophysin-positive neurons quantified in adult zebrafish.The European journal of neuroscience · 2018Article
- Probiotic modulation of the microbiota-gut-brain axis and behaviour in zebrafish.Scientific reports · 2016Article
- BDNF Expression in Larval and Adult Zebrafish Brain: Distribution and Cell Identification.PloS one · 2016Article
- Zebrafish: a model for the study of addiction genetics.Human genetics · 2012Review
- Brain-derived neurotrophic factor mediates non-cell-autonomous regulation of sensory neuron position and identity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Expression of brain-derived neurotrophic factor and TrkB in the lateral line system of zebrafish during development.Cellular and molecular neurobiology · 2010Article
- Brain-derived neurotrophic factor and the development of structural neuronal connectivity.Developmental neurobiology · 2010Review
- Acute stress alters transcript expression pattern and reduces processing of proBDNF to mature BDNF in Dicentrarchus labrax.BMC neuroscience · 2010Article
- Mouse and rat BDNF gene structure and expression revisited.Journal of neuroscience research · 2007Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPrecise control of developmental and cell-specific expression of the brain-derived neurotrophic factor (BDNF) gene is essential for normal neuronal development and the diverse functions of BDNF in the adult organism. We previously showed that the zebrafish BDNF gene has multiple promoters. The complexity of the promoter structure and the mechanisms that mediate developmental and cell-specific expression are still incompletely understood.
resultsComparison of pufferfish and zebrafish BDNF gene sequences as well as 5' RACE revealed three additional 5' exons and associated promoters. RT-PCR with exon-specific primers showed differential developmental and organ-specific expression. Two exons were detected in the embryo before transcription starts. Of the adult organs examined, the heart expressed a single 5' exon whereas the brain, liver and eyes expressed four of the seven 5' exons. Three of the seven 5' exons were not detectable by RT-PCR. Injection of promoter/GFP constructs into embryos revealed distinct expression patterns. The 3' flank profoundly affected expression in a position-dependent manner and a highly conserved sequence (HCS1) present in 5' exon 1c in a dehancer-like manner.
conclusionsThe zebrafish BDNF gene is as complex in its promoter structure and patterns of differential promoter expression as is its murine counterpart. The expression of two of the promoters appears to be regulated in a temporally and/or spatially highly circumscribed fashion. The 3' flank has a position-dependent effect on expression, either by affecting transcription termination or post-transcriptional steps. HCS1, a highly conserved sequence in 5' exon 1c, restricts expression to primary sensory neurons. The tools are now available for detailed genetic and molecular analyses of zebrafish BDNF gene expression.
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