ArticleThe Biochemical journal2004
A co-translational model to explain the in vivo import of proteins into HeLa cell mitochondria.
Article in The Biochemical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 52 citations in OpenAlex.
- Endogenous TOM20 Proximity Labeling: A Swiss-Knife for the Study of Mitochondrial Proteins in Human Cells.International journal of molecular sciences · 2023Article
- Computational Evaluation of Peroxisomal Targeting Signals in Metazoa.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Mitochondrial mRNA localization is governed by translation kinetics and spatial transport.PLoS computational biology · 2022Article
- Article
- mRNA Targeting, Transport and Local Translation in Eukaryotic Cells: From the Classical View to a Diversity of New Concepts.Molecular biology · 2021Article
- Organelle-associated rRNA Degradation.Bio-protocol · 2019Article
- Regulation of mitochondrion-associated cytosolic ribosomes by mammalian mitochondrial ribonuclease T2 (RNASET2).The Journal of biological chemistry · 2018Article
- Amyloid β-peptides interfere with mitochondrial preprotein import competence by a coaggregation process.Molecular biology of the cell · 2016Article
- Antibiotic use and abuse: a threat to mitochondria and chloroplasts with impact on research, health, and environment.BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Article
- Localized translation near the mitochondrial outer membrane: An update.RNA biology · 2015Review
- The similarity between N-terminal targeting signals for protein import into different organelles and its evolutionary relevance.Frontiers in physiology · 2015Review
- Mitochondrial medicine: to a new era of gene therapy for mitochondrial DNA mutations.Journal of inherited metabolic disease · 2011Review
- Mitochondrial translation and beyond: processes implicated in combined oxidative phosphorylation deficiencies.Journal of biomedicine & biotechnology · 2010Review
- Metal acquisition and availability in the mitochondria.Chemical reviews · 2009Review
- A point mutation produced a class 3 aldehyde dehydrogenase with increased protective ability against the killing effect of cyclophosphamide.Biochemical pharmacology · 2008Article
- Mitochondrial nuclear receptors and transcription factors: who's minding the cell?Journal of neuroscience research · 2008Review
- mRNA trafficking in fungi.Molecular genetics and genomics : MGG · 2007Review
- Delivery of drugs and macromolecules to mitochondria.Advanced drug delivery reviews · 2007Review
- Mitochondrial protein import and human health and disease.Biochimica et biophysica acta · 2007Review
- Mitochondria-associated yeast mRNAs and the biogenesis of molecular complexes.Molecular biology of the cell · 2007Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The dual signal approach, i.e. a mitochondrial signal at the N-terminus and an ER (endoplasmic reticulum) or a peroxisomal signal at the C-terminus of EGFP (enhanced green fluorescent protein), was employed in transfected HeLa cells to test for a co-translational import model. The signal peptide from OTC (ornithine transcarbamylase) or arginase II was fused to the N-terminus of EGFP, and an ER or peroxisomal signal was fused to its C-terminus. The rationale was that if the free preprotein remained in the cytosol, it could be distributed between the two organelles by using a post-translational pathway. The resulting fusion proteins were imported exclusively into mitochondria, suggesting that co-translational import occurred. Native preALDH (precursor of rat liver mitochondrial aldehyde dehydrogenase), preOTC and rhodanese, each with the addition of a C-terminal ER or peroxisomal signal, were also translocated only to the mitochondria, again showing that a co-translational import pathway exists for these native proteins. Import of preALDH(sp)-DHFR, a fusion protein consisting of the leader sequence (signal peptide) of preALDH fused to DHFR (dihydrofolate reductase), was studied in the presence of methotrexate, a substrate analogue for DHFR. It was found that 70% of the preALDH(sp)-DHFR was imported into mitochondria in the presence of methotrexate, implying that 70% of the protein utilized the co-translational import pathway and 30% used the post-translational import pathway. Thus it appears that co-translational import is a major pathway for mitochondrial protein import. A model is proposed to explain how competition between binding factors could influence whether or not a cytosolic carrier protein, such as DHFR, uses the co- or post-translational import pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.