ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2004
Syntaxin-1A is excluded from recycling synaptic vesicles at nerve terminals.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 57 citations in OpenAlex.
- Cocaine-regulated trafficking of dopamine transporters in cultured neurons revealed by a pH sensitive reporter.iScience · 2023Article
- Quantitative Analysis of Presynaptic Vesicle Luminal pH in Cultured Neurons.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Opponent vesicular transporters regulate the strength of glutamatergic neurotransmission in a C. elegans sensory circuit.Nature communications · 2021Article
- The endosomal Q-SNARE, Syntaxin 7, defines a rapidly replenishing synaptic vesicle recycling pool in hippocampal neurons.Communications biology · 2021Article
- The mechanism and regulation of vesicular glutamate transport: Coordination with the synaptic vesicle cycle.Biochimica et biophysica acta. Biomembranes · 2020Review
- Expression of plasma membrane calcium ATPases confers CaScientific reports · 2019Article
- A mouse model of autism implicates endosome pH in the regulation of presynaptic calcium entry.Nature communications · 2018Article
- Tomosyn associates with secretory vesicles in neurons through its N- and C-terminal domains.PloS one · 2017Article
- Article
- Monitoring of vacuolar-type H+ ATPase-mediated proton influx into synaptic vesicles.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Munc18-1 redistributes in nerve terminals in an activity- and PKC-dependent manner.The Journal of cell biology · 2014Article
- Article
- Syntaxin1A lateral diffusion reveals transient and local SNARE interactions.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Article
- AP180 and CALM: Dedicated endocytic adaptors for the retrieval of synaptobrevin 2 at synapses.Cellular logistics · 2011Article
- Vesicular sterols are essential for synaptic vesicle cycling.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Endosomal sorting of readily releasable synaptic vesicles.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Vesicular monoamine and glutamate transporters select distinct synaptic vesicle recycling pathways.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Vesicular glutamate transport promotes dopamine storage and glutamate corelease in vivo.Neuron · 2010Article
- Synaptic vesicle pools: an update.Frontiers in synaptic neuroscience · 2010Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
At presynaptic terminals, intermixing during cycles of exocytosis and endocytosis challenges the molecular identity of the plasma and synaptic vesicle membranes. Although synaptic vesicle components are retrieved during recycling, the extent to which plasma membrane proteins enter the synaptic vesicle recycling pathway has not been examined. The target-SNARE (N-ethylmaleimide-sensitive factor attachment protein receptor) syntaxin-1 was shown previously to be present on putative synaptic vesicular membranes (Koh et al., 1993; Walch-Solimena et al., 1995; Kretzschmar et al., 1996), suggesting that syntaxin may cycle between the synaptic vesicle pool and the cell surface (Walch-Solimena et al., 1995). This implies that the molecular identity of the two membranes is not maintained during synaptic activity. Because the main role of syntaxin-1 is as a target-SNARE for vesicle fusion, appearance on synaptic vesicles could lead to futile interactions with vesicle-SNARE proteins. We investigated whether the subcellular localization of syntaxin-1A, tagged with the pH-sensitive fluorescent tag pHluorin, is regulated during neurotransmission using laser-scanning microscopy. We report here that syntaxin-1A is predominantly localized to the plasma membrane, with a small proportion present in an intracellular compartment with a lumenal pH consistent with synaptic vesicles. However, the internal fraction of syntaxin-1A is excluded from synaptic vesicles that undergo action potential-dependent recycling. These data indicate that the molecular identity of opposing exocytotic membranes is preserved by the sorting of syntaxin-1A from recycling synaptic vesicles.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.