ArticleJournal of molecular medicine (Berlin, Germany)2004
Mammalian cell transduction and internalization properties of lambda phages displaying the full-length adenoviral penton base or its central domain.
Article in Journal of molecular medicine (Berlin, Germany), 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 40 citations in OpenAlex.
- Virus-like particles based on plant viruses and bacteriophages: emerging strategies for the delivery of nucleic acid therapeutics.Chemical science · 2026Review
- Bacteriophage T4 as a nanovehicle for delivery of genes and therapeutics into human cells.Current opinion in virology · 2022Review
- Evaluation of dendritic cell-targeting T7 phages as a vehicle to deliver avian influenza virus H5 DNA vaccine in SPF chickens.Frontiers in immunology · 2022Article
- Engineering T7 bacteriophage as a potential DNA vaccine targeting delivery vector.Virology journal · 2018Article
- Bacteriophages and phage-inspired nanocarriers for targeted delivery of therapeutic cargos.Advanced drug delivery reviews · 2016Review
- Hybrid Nanomaterial Complexes for Advanced Phage-guided Gene Delivery.Molecular therapy. Nucleic acids · 2014Article
- Bacteriophages and medical oncology: targeted gene therapy of cancer.Medical oncology (Northwood, London, England) · 2014Review
- Synthetic phage for tissue regeneration.Mediators of inflammation · 2014Review
- Targeted delivery system of nanobiomaterials in anticancer therapy: from cells to clinics.BioMed research international · 2014Review
- Clathrin-mediated endocytosis and subsequent endo-lysosomal trafficking of adeno-associated virus/phage.The Journal of biological chemistry · 2012Article
- Bacteriophages and their implications on future biotechnology: a review.Virology journal · 2012Review
- Article
- AAV's anatomy: roadmap for optimizing vectors for translational success.Current gene therapy · 2010Article
- Biological gene delivery vehicles: beyond viral vectors.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Review
- Gene transfer of Hodgkin cell lines via multivalent anti-CD30 scFv displaying bacteriophage.BMC molecular biology · 2008Article
- A tractable method for simultaneous modifications to the head and tail of bacteriophage lambda and its application to enhancing phage-mediated gene delivery.Nucleic acids research · 2007Article
- Vascular targeting: recent advances and therapeutic perspectives.Trends in cardiovascular medicine · 2006Review
- A simple method for displaying recalcitrant proteins on the surface of bacteriophage lambda.Nucleic acids research · 2005Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In recent years a strong effort has been devoted to the search for new, safe and efficient gene therapy vectors. Phage lambda is a promising backbone for the development of new vectors: its genome can host large inserts, DNA is protected from degradation by the capsid and the ligand-exposed D and V proteins can be extensively modified. Current phage-based vectors are inefficient and/or receptor-independent transducers. To produce new, receptor-selective and transduction-efficient vectors for mammalian cells we engineered lambda by inserting into its genome a GFP expression cassette, and by displaying the penton base (Pb) of adenovirus or its central region (amino acids 286-393). The Pb mediates attachment, entry and endosomal escape of adenovirus in mammalian cells, and its central region (amino acids 286-393) includes the principal receptor-binding motif ((340)RGD(342)). Both the phage chimerae lambda Pb and lambda Pb (286-393) were able to transduce cell lines and primary cultures of human fibroblasts. Competition experiments showed that the transduction pathway was receptor-dependent. We also describe the different trafficking properties of lambda Pb and lambda Pb (286-393). Bafilomycin, which blocks endosome maturation, influenced the intracellular distribution of lambda Pb (286-393), but not that of lambda Pb. The proteasome inhibitor MG-132 improved the efficiency of lambda Pb (286-393)-mediated transduction, but not that of lambda Pb. In summary, this work shows the feasibility of using lambda phage as an efficient vector for gene transfer into mammalian cells. We show that lambda Pb and lambda Pb (286-393) can both mediate receptor-dependent transduction; while only lambda Pb is able to promote endosomal escape and proteasome resistance of phage particles.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.