ArticleJournal of bacteriology2004
The Sinorhizobium meliloti fur gene regulates, with dependence on Mn(II), transcription of the sitABCD operon, encoding a metal-type transporter.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 77 citations in OpenAlex.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Bacterial iron detoxification at the molecular level.The Journal of biological chemistry · 2020Review
- Multiple Sense and Antisense Promoters Contribute to the Regulated Expression of theMicroorganisms · 2019Article
- Article
- Manganese transport is essential for NEnvironmental microbiology · 2017Article
- Prokaryotic Heme Biosynthesis: Multiple Pathways to a Common Essential Product.Microbiology and molecular biology reviews : MMBR · 2017Review
- Metal-specific control of gene expression mediated by Bradyrhizobium japonicum Mur and Escherichia coli Fur is determined by the cellular context.Molecular microbiology · 2016Article
- Discrete Responses to Limitation for Iron and Manganese in Agrobacterium tumefaciens: Influence on Attachment and Biofilm Formation.Journal of bacteriology · 2015Article
- Manganese uptake and streptococcal virulence.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2015Review
- Magnesium-dependent processes are targets of bacterial manganese toxicity.Molecular microbiology · 2014Article
- The LysR-type transcription factor HbrL is a global regulator of iron homeostasis and porphyrin synthesis in Rhodobacter capsulatus.Molecular microbiology · 2013Article
- Manganese uptake in marine bacteria; the novel MntX transporter is widespread in Roseobacters, Vibrios, Alteromonadales and the SAR11 and SAR116 clades.The ISME journal · 2013Article
- Iron homeostasis in theAdvances in botanical research · 2013Article
- Manganese is required for oxidative metabolism in unstressed Bradyrhizobium japonicum cells.Molecular microbiology · 2012Article
- Mur regulates the gene encoding the manganese transporter MntH in Brucella abortus 2308.Journal of bacteriology · 2012Article
- Role of the Irr protein in the regulation of iron metabolism in Rhodobacter sphaeroides.PloS one · 2012Article
- The Fur regulon in anaerobically grown Salmonella enterica sv. Typhimurium: identification of new Fur targets.BMC microbiology · 2011Article
- Bacterial outer membrane channel for divalent metal ion acquisition.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Exploring the symbiotic pangenome of the nitrogen-fixing bacterium Sinorhizobium meliloti.BMC genomics · 2011Article
- Transcriptional control of the Bradyrhizobium japonicum irr gene requires repression by fur and Antirepression by Irr.The Journal of biological chemistry · 2010Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sinorhizobium meliloti is an alpha-proteobacterium able to induce nitrogen-fixing nodules on roots of specific legumes. In order to propagate in the soil and for successful symbiotic interaction the bacterium needs to sequester metals like iron and manganese from its environment. The metal uptake has to be in turn tightly regulated to avoid toxic effects. In this report we describe the characterization of a chromosomal region of S. meliloti encoding the sitABCD operon and the putative regulatory fur gene. It is generally assumed that the sitABCD operon encodes a metal-type transporter and that the fur gene is involved in iron ion uptake regulation. A constructed S. meliloti sitA deletion mutant was found to be growth dependent on Mn(II) and to a lesser degree on Fe(II). The sitA promoter was strongly repressed by Mn(II), with dependence on Fur, and moderately by Fe(II). Applying a genome-wide S. meliloti microarray it was shown that in the fur deletion mutant 23 genes were up-regulated and 10 genes were down-regulated when compared to the wild-type strain. Among the up-regulated genes only the sitABCD operon could be associated with metal uptake. On the other hand, the complete rhbABCDEF operon, which is involved in siderophore synthesis, was identified among the down-regulated genes. Thus, in S. meliloti Fur is not a global repressor of iron uptake. Under symbiotic conditions the sitA promoter was strongly expressed and the S. meliloti sitA mutant exhibited an attenuated nitrogen fixation activity resulting in a decreased fresh weight of the host plant Medicago sativa.
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