ArticleJournal of bacteriology2004
Transcription by an archaeal RNA polymerase is slowed but not blocked by an archaeal nucleosome.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 58 citations in OpenAlex.
- Chromatin and gene regulation in archaea.Molecular microbiology · 2025Review
- Archaeal histone-based chromatin structures regulate transcription elongation rates.Communications biology · 2024Article
- The chromatin landscape of the euryarchaeon Haloferax volcanii.Genome biology · 2023Article
- DNA-bridging by an archaeal histone variant via a unique tetramerisation interface.Communications biology · 2023Article
- Article
- An archaeal histone-like protein regulates gene expression in response to salt stress.Nucleic acids research · 2021Article
- The hyperthermophilic archaeon Thermococcus kodakarensis is resistant to pervasive negative supercoiling activity of DNA gyrase.Nucleic acids research · 2021Article
- Archaeal chromatin 'slinkies' are inherently dynamic complexes with deflected DNA wrapping pathways.eLife · 2021Article
- The DNA-binding protein HTa fromeLife · 2019Article
- Chromatinization ofeLife · 2019Article
- The Role of Archaeal Chromatin in Transcription.Journal of molecular biology · 2019Review
- Old cogs, new tricks: the evolution of gene expression in a chromatin context.Nature reviews. Genetics · 2019Review
- TFS and Spt4/5 accelerate transcription through archaeal histone-based chromatin.Molecular microbiology · 2019Article
- Structure and function of archaeal histones.PLoS genetics · 2018Review
- Archaeal RNA polymerase arrests transcription at DNA lesions.Transcription · 2017Article
- Transcription Regulation in Archaea.Journal of bacteriology · 2016Review
- The oligomeric architecture of the archaeal exosome is important for processive and efficient RNA degradation.Nucleic acids research · 2016Article
- Mechanisms of Evolutionary Innovation Point to Genetic Control Logic as the Key Difference Between Prokaryotes and Eukaryotes.Journal of molecular evolution · 2015Article
- The interplay between nucleoid organization and transcription in archaeal genomes.Nature reviews. Microbiology · 2015Review
- Chromatin structure and dynamics in hot environments: architectural proteins and DNA topoisomerases of thermophilic archaea.International journal of molecular sciences · 2014Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Archaeal RNA polymerases (RNAPs) are closely related to eukaryotic RNAPs, and in Euryarchaea, genomic DNA is wrapped and compacted by histones into archaeal nucleosomes. In eukaryotes, transcription of DNA bound into nucleosomes is facilitated by histone tail modifications and chromatin remodeling complexes, but archaeal histones do not have histone tails and archaeal genome sequences provide no evidence for archaeal homologs of eukaryotic chromatin remodeling complexes. We have therefore investigated the ability of an archaeal RNAP, purified from Methanothermobacter thermautotrophicus, to transcribe DNA bound into an archaeal nucleosome by HMtA2, an archaeal histone from M. thermautotrophicus. To do so, we constructed a template that allows transcript elongation to be separated from transcription initiation, on which archaeal nucleosome assembly is positioned downstream from the site of transcription initiation. At 58 degrees C, in the absence of an archaeal nucleosome, M. thermautotrophicus RNAP transcribed this template DNA at a rate of approximately 20 nucleotides per second. With an archaeal nucleosome present, transcript elongation was slowed but not blocked, with transcription pausing at sites before and within the archaeal nucleosome. With additional HMtA2 binding, complexes were obtained that also incorporated the upstream regulatory region. This inhibited transcription presumably by preventing archaeal TATA-box binding protein, general transcription factor TFB, and RNAP access and thus inhibiting transcription initiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.