ArticleJournal of bacteriology2004
The spatial organization of the VirR boxes is critical for VirR-mediated expression of the perfringolysin O gene, pfoA, from Clostridium perfringens.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Identifying the Basis for VirS/VirR Two-Component Regulatory System Control of Clostridium perfringens Beta-Toxin Production.Journal of bacteriology · 2021Article
- Review
- RNA-seq analysis of virR and revR mutants of Clostridium perfringens.BMC genomics · 2016Article
- Article
- Review
- Article
- The Pseudomonas aeruginosa AlgZR two-component system coordinates multiple phenotypes.Frontiers in cellular and infection microbiology · 2014Review
- Toxin plasmids of Clostridium perfringens.Microbiology and molecular biology reviews : MMBR · 2013Review
- Burkholderia xenovorans RcoM(Bx)-1, a transcriptional regulator system for sensing low and persistent levels of carbon monoxide.Journal of bacteriology · 2012Article
- A novel toxin regulator, the CPE1446-CPE1447 protein heteromeric complex, controls toxin genes in Clostridium perfringens.Journal of bacteriology · 2011Article
- Article
- The VirSR two-component signal transduction system regulates NetB toxin production in Clostridium perfringens.Infection and immunity · 2010Article
- Identification of novel pathogenicity loci in Clostridium perfringens strains that cause avian necrotic enteritis.PloS one · 2010Article
- Comparative genomics of VirR regulons in Clostridium perfringens strains.BMC microbiology · 2010Article
- Article
- Contact with enterocyte-like Caco-2 cells induces rapid upregulation of toxin production by Clostridium perfringens type C isolates.Cellular microbiology · 2009Article
- Use of an EZ-Tn5-based random mutagenesis system to identify a novel toxin regulatory locus in Clostridium perfringens strain 13.PloS one · 2009Article
- Article
- Characterization of genes regulated directly by the VirR/VirS system in Clostridium perfringens.Journal of bacteriology · 2008Article
- plcR papR-independent expression of anthrolysin O by Bacillus anthracis.Journal of bacteriology · 2006Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transcriptional regulation of toxin production in the gram-positive anaerobe Clostridium perfringens involves a two-component signal transduction system that comprises the VirS sensor histidine kinase and its cognate response regulator, VirR. Previous studies showed that VirR binds independently to a pair of imperfect direct repeats, now designated VirR box 1 and VirR box 2, located immediately upstream of the promoter of the pfoA gene, which encodes the cholesterol-dependent cytolysin, perfringolysin O. For this study, we introduced mutated VirR boxes into a C. perfringens pfoA mutant and found that both VirR boxes are essential for transcriptional activation. Furthermore, the spacing between the VirR boxes and the distance between the VirR boxes and the -35 region are shown to be critical for perfringolysin O production. Other VirR boxes that were previously identified from the strain 13 genome sequence were also analyzed, with perfringolysin O production used as a reporter system. The results showed that placement of the different VirR boxes at the same position upstream of the pfoA promoter yields different levels of perfringolysin O activity. In all of these constructs, VirR was still capable of binding to the target DNA, indicating that DNA binding alone is not sufficient for transcriptional activation. Finally, we show that the C. perfringens RNA polymerase binds more efficiently to the pfoA promoter in the presence of VirR, indicating that interactions must occur between these proteins. We propose that these interactions are required for VirR-mediated transcriptional activation.
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