ArticleThe Biochemical journal2004
Transketolase from Leishmania mexicana has a dual subcellular localization.
Article in The Biochemical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 54 citations in OpenAlex.
- Transketolase-mediated erythrose-4-phosphate provides an essential source for anthocyanin biosynthesis in petunia.Horticulture research · 2025Article
- The mechanism of a one-substrate transketolase reaction.Bioscience reports · 2020Article
- A 'Split-Gene' Transketolase From the Hyper-Thermophilic BacteriumFrontiers in microbiology · 2020Article
- Deletion of transketolase triggers a stringent metabolic response in promastigotes and loss of virulence in amastigotes of Leishmania mexicana.PLoS pathogens · 2018Article
- Article
- The Potential of Secondary Metabolites from Plants as Drugs or Leads against Protozoan Neglected Diseases-Part III: In-Silico Molecular Docking Investigations.Molecules (Basel, Switzerland) · 2016Review
- Disclosing the essentiality of ribose-5-phosphate isomerase B in Trypanosomatids.Scientific reports · 2016Article
- High-resolution structures of Lactobacillus salivarius transketolase in the presence and absence of thiamine pyrophosphate.Acta crystallographica. Section F, Structural biology communications · 2015Article
- The return of metabolism: biochemistry and physiology of the pentose phosphate pathway.Biological reviews of the Cambridge Philosophical Society · 2015Review
- Decreased TK activity alters growth, yield and tolerance to low temperature and low light intensity in transgenic cucumber plants.Plant cell reports · 2015Article
- Characterization of two transketolases encoded on the chromosome and the plasmid pBM19 of the facultative ribulose monophosphate cycle methylotroph Bacillus methanolicus.BMC microbiology · 2014Article
- Thiol redox sensitivity of two key enzymes of heme biosynthesis and pentose phosphate pathways: uroporphyrinogen decarboxylase and transketolase.Oxidative medicine and cellular longevity · 2013Article
- Structure and function of the transketolase from Mycobacterium tuberculosis and comparison with the human enzyme.Open biology · 2012Article
- Expression, crystallization and preliminary X-ray crystallographic analysis of glucose-6-phosphate dehydrogenase from the human pathogen Trypanosoma cruzi in complex with substrate.Acta crystallographica. Section F, Structural biology and crystallization communications · 2011Article
- Revealing the functions of the transketolase enzyme isoforms in Rhodopseudomonas palustris using a systems biology approach.PloS one · 2011Article
- Glucose-6-phosphate dehydrogenase of trypanosomatids: characterization, target validation, and drug discovery.Molecular biology international · 2011Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transketolase has been characterized in Leishmania mexicana. A gene encoding this enzyme was identified and cloned. The gene was expressed in Escherichia coli and the protein was purified and characterized. An apparent K(m) of 2.75 mM for ribose 5-phosphate was determined. X-ray crystallography was used to determine the three-dimensional structure of the enzyme to a resolution of 2.2 A (1 A identical with 0.1 nm). The C-terminus of the protein contains a type-1 peroxisome-targeting signal, suggestive of a possible glycosomal subcellular localization. Subcellular localization experiments performed with promastigote forms of the parasite revealed that the protein was predominantly cytosolic, although a significant component of the total activity was associated with the glycosomes. Transketolase is thus the first enzyme of the nonoxidative branch of the pentose phosphate pathway whose presence has been demonstrated in a peroxisome-like organelle.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.