ArticleProceedings of the National Academy of Sciences of the United States of America2004
Deletion of the Parkin coregulated gene causes male sterility in the quaking(viable) mouse mutant.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed, 94 citations in OpenAlex.
- Evolutionary adaptations of doublet microtubules in trypanosomatid parasites.Science (New York, N.Y.) · 2025Article
- Article
- Article
- Whole-genome methylation analysis of testicular germ cells from cryptozoospermic men points to recurrent and functionally relevant DNA methylation changes.Clinical epigenetics · 2021Article
- Exome sequencing reveals variants in known and novel candidate genes for severe sperm motility disorders.Human reproduction (Oxford, England) · 2021Article
- Crystal structure of human PACRG in complex with MEIG1 reveals roles in axoneme formation and tubulin binding.Structure (London, England : 1993) · 2021Article
- HAG1 and SWI3A/B control of male germ line development in P. patens suggests conservation of epigenetic reproductive control across land plants.Plant reproduction · 2021Article
- Functional Expression, Purification and Identification of Interaction Partners of PACRG.Molecules (Basel, Switzerland) · 2021Article
- Elucidating the processes and pathways enriched in buffalo sperm proteome in regulating semen quality.Cell and tissue research · 2021Article
- TheScience signaling · 2020Article
- Article
- Mechanisms of PINK1, ubiquitin and Parkin interactions in mitochondrial quality control and beyond.Cellular and molecular life sciences : CMLS · 2019Review
- Article
- PACRG and FAP20 form the inner junction of axonemal doublet microtubules and regulate ciliary motility.Molecular biology of the cell · 2019Article
- Transcription factor DUO1 generated by neo-functionalization is associated with evolution of sperm differentiation in plants.Nature communications · 2018Article
- Generation and characterisation of a parkin-Pacrg knockout mouse line and a Pacrg knockout mouse line.Scientific reports · 2018Article
- Microtubule binding protein PACRG plays a role in regulating specific ciliary dyneins during microtubule sliding.Cytoskeleton (Hoboken, N.J.) · 2016Article
- Article
- PACRG, a protein linked to ciliary motility, mediates cellular signaling.Molecular biology of the cell · 2016Article
- Sperm dysfunction and ciliopathy.Reproductive medicine and biology · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Quaking(viable) (qk(v)) is a recessive neurological mouse mutation with severe dysmyelination of the CNS and spermiogenesis failure. The molecular lesion in the qk(v) mutant is a deletion of approximately 1 Mb on mouse chromosome 17 that alters the expression of the qk gene in oligodendrocytes. Complementation analysis between the qk(v) mutation and qk mutant alleles generated through chemical mutagenesis showed that the male sterility is a distinctive feature of the qk(v) allele. This observation suggested that the sperm differentiation defect in qk(v) is due to the deletion of a gene(s) distinct from qk. Here, we demonstrate that the deletion of Pacrg is the cause of male sterility in the qk(v) mutant. Pacrg is the mouse homologue of the human PARKIN-coregulated gene (PACRG), which encodes for a protein whose biochemical function remains unclear. We show that Pacrg is highly expressed in the testes in both mice and humans. In addition, the expression pattern of Pacrg during spermiogenesis suggests that it plays a role in sperm differentiation. In support of this hypothesis, we show that transgenic expression of Pacrg in testes restores spermiogenesis and fertility in qk(v) males. This finding provides the first in vivo evidence, to our knowledge, for the function of Pacrg in a model organism. Immunolocalization experiments on isolated spermatozoa show that the Pacrg protein is present in mature sperm. Remarkably, the mammalian Pacrg protein shares significant sequence similarities with gene products from flagellated protozoans, suggesting that Pacrg may be necessary for proper flagellar formation in many organisms.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.