ArticleProceedings of the National Academy of Sciences of the United States of America2004
Scanning electrochemical microscopy of menadione-glutathione conjugate export from yeast cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Glutathione Relevance in the Response of CiliateBiology · 2026Article
- Investigating the cytotoxic redox mechanism of PFOS within Hep G2 by hyperspectral-assisted scanning electrochemical microscopy.The Analyst · 2022Article
- Recent Advances in Scanning Electrochemical Microscopy for Biological Applications.Materials (Basel, Switzerland) · 2018Review
- Different characteristics between menadione and menadione sodium bisulfite as redox mediator in yeast cell suspension.Biochemistry and biophysics reports · 2016Article
- Electrochemical detection of intracellular and cell membrane redox systems in Saccharomyces cerevisiae.Scientific reports · 2014Article
- Assessment of multidrug resistance on cell coculture patterns using scanning electrochemical microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Brazilian propolis protects Saccharomyces cerevisiae cells against oxidative stress.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2013Article
- Inhibition of the MRP1-mediated transport of the menadione-glutathione conjugate (thiodione) in HeLa cells as studied by SECM.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Glutathione transferases of Phanerochaete chrysosporium: S-glutathionyl-p-hydroquinone reductase belongs to a new structural class.The Journal of biological chemistry · 2011Article
- Quantitative visualization of passive transport across bilayer lipid membranes.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- The Kv channel blocker 4-aminopyridine enhances Ag+ uptake: a scanning electrochemical microscopy study of single living cells.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Nanoelectrochemistry of mammalian cells.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Menadione metabolism to thiodione in hepatoblastoma by scanning electrochemical microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Scanning electrochemical microscopy: detection of human breast cancer cells by redox environment.Journal of mammary gland biology and neoplasia · 2004Review
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2 authors.
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Abstract
The uptake of menadione (2-methyl-1,4-naphthoquinone), which is toxic to yeast cells, and its expulsion as a glutathione complex were studied by scanning electrochemical microscopy. The progression of the in vitro reaction between menadione and glutathione was monitored electrochemically by cyclic voltammetry and correlated with the spectroscopic (UV-visible) behavior. By observing the scanning electrochemical microscope tip current of yeast cells suspended in a menadione-containing solution, the export of the conjugate from the cells with time could be measured. Similar experiments were performed on immobilized yeast cell aggregates stressed by a menadione solution. From the export of the menadione-glutathione conjugate detected at a 1-microm-diameter electrode situated 10 microm from the cells, a flux of about 30,000 thiodione molecules per second per cell was extracted. Numerical simulations based on an explicit finite difference method further revealed that the observation of a constant efflux of thiodione from the cells suggested the rate was limited by the uptake of menadione and that the efflux through the glutathione-conjugate pump was at least an order of magnitude faster.
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