ArticleMolecular biology of the cell2004
Disruption of astral microtubule contact with the cell cortex activates a Bub1, Bub3, and Mad3-dependent checkpoint in fission yeast.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 49 citations in OpenAlex.
- MAARS Software for Automatic and Quantitative Analysis of Mitotic Progression.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Unraveling the kinetochore nanostructure in Schizosaccharomyces pombe using multi-color SMLM imaging.The Journal of cell biology · 2023Article
- Force by minus-end motors Dhc1 and Klp2 collapses the S. pombe spindle after laser ablation.Biophysical journal · 2022Article
- Super-resolved live-cell imaging using random illumination microscopy.Cell reports methods · 2021Article
- Escape from mitotic catastrophe by actin-dependent nuclear displacement in fission yeast.iScience · 2021Article
- Article
- Aurora B and condensin are dispensable for chromosome arm and telomere separation during meiosis II.Molecular biology of the cell · 2020Article
- Nuclear envelope attachment of telomeres limits TERRA and telomeric rearrangements in quiescent fission yeast cells.Nucleic acids research · 2020Article
- The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.The Journal of cell biology · 2017Article
- MAARS: a novel high-content acquisition software for the analysis of mitotic defects in fission yeast.Molecular biology of the cell · 2017Article
- Fission yeast kinesin-8 controls chromosome congression independently of oscillations.Journal of cell science · 2015Article
- Aurora B prevents chromosome arm separation defects by promoting telomere dispersion and disjunction.The Journal of cell biology · 2015Article
- The novel actin/focal adhesion-associated protein MISP is involved in mitotic spindle positioning in human cells.Cell cycle (Georgetown, Tex.) · 2013Article
- Mammalian HCA66 protein is required for both ribosome synthesis and centriole duplication.Nucleic acids research · 2012Article
- A stochastic model of kinetochore-microtubule attachment accurately describes fission yeast chromosome segregation.The Journal of cell biology · 2012Article
- And the dead shall rise: actin and myosin return to the spindle.Developmental cell · 2011Review
- Tip1/CLIP-170 protein is required for correct chromosome poleward movement in fission yeast.PloS one · 2010Article
- Ase1/Prc1-dependent spindle elongation corrects merotely during anaphase in fission yeast.The Journal of cell biology · 2009Article
- Cyclin A/cdk2 regulates adenomatous polyposis coli-dependent mitotic spindle anchoring.The Journal of biological chemistry · 2009Article
- Dual regulation of Mad2 localization on kinetochores by Bub1 and Dam1/DASH that ensure proper spindle interaction.Molecular biology of the cell · 2008Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
In animal and yeast cells, the mitotic spindle is aligned perpendicularly to the axis of cell division. This ensures that sister chromatids are separated to opposite sides of the cytokinetic actomyosin ring. In fission yeast, spindle rotation is dependent upon the interaction of astral microtubules with the cortical actin cytoskeleton. In this article, we show that addition of Latrunculin A, which prevents spindle rotation, delays the separation of sister chromatids and anaphase promoting complex-mediated destruction of spindle-associated Securin and Cyclin B. Moreover, we find that whereas sister kinetochore pairs normally congress to the spindle midzone before anaphase onset, this congression is disrupted when astral microtubule contact with the actin cytoskeleton is disturbed. By analyzing the timing of kinetochore separation, we find that this anaphase delay requires the Bub3, Mad3, and Bub1 but not the Mad1 or Mad2 spindle assembly checkpoint proteins. In agreement with this, we find that Bub1 remains associated with kinetochores when spindles are mispositioned. These data indicate that, in fission yeast, astral microtubule contact with the medial cell cortex is monitored by a subset of spindle assembly checkpoint proteins. We propose that this checkpoint ensures spindles are properly oriented before anaphase takes place.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.