ArticleMolecular biology of the cell2004
Cell cycle-dependent regulation of a human DNA helicase that localizes in DNA damage foci.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 71 citations in OpenAlex.
- Discovering novel SNPs that are correlated with patient outcome in a Singaporean cancer patient cohort treated with gemcitabine-based chemotherapy.BMC cancer · 2018Trial
- A Constellation of Fluorescent Biosensors to Illuminate the Galaxy of Protein Kinases.Chembiochem : a European journal of chemical biology · 2026Review
- Direct Mapping of CDK2 Substrates in Embryonic Stem Cells Uncovers an AP-Site Repair Mechanism via HMCES Phosphorylation.bioRxiv : the preprint server for biology · 2026Article
- Quantitative CDK2 Dynamics Are Linked to Cell Fate Decisions in Differentiating Trophoblast Stem Cells.bioRxiv : the preprint server for biology · 2026Article
- Genetic variants in HELB contribute to premature ovarian insufficiency and early age of natural menopause.JCI insight · 2025Article
- Rare SNP in theNAR molecular medicine · 2025Article
- CDK2 activity crosstalk on the ERK kinase translocation reporter can be resolved computationally.Cell systems · 2025Article
- A concise guide to fluorescent cell cycle reporters for live-cell imaging.Frontiers in cell and developmental biology · 2025Review
- The role of RAD51 regulators and variants in primary ovarian insufficiency, endometriosis, and polycystic ovary syndrome.NAR molecular medicine · 2024Review
- Article
- The TRESLIN-MTBP complex couples completion of DNA replication with S/G2 transition.Molecular cell · 2022Article
- CDK activity sensors: genetically encoded ratiometric biosensors for live analysis of the cell cycle.Biochemical Society transactions · 2022Article
- Quantitative profiling of adaptation to cyclin E overproduction.Life science alliance · 2022Article
- Experimental and bioinformatics considerations in cancer application of single cell genomics.Computational and structural biotechnology journal · 2021Article
- SCFCell death & disease · 2020Article
- The Expression of Human DNA Helicase B Is Affected by G-Quadruplexes in the Promoter.Biochemistry · 2020Article
- Genome Maintenance by DNA Helicase B.Genes · 2020Review
- Transient Hysteresis in CDK4/6 Activity Underlies Passage of the Restriction Point in G1.Molecular cell · 2019Article
- Signalling involving MET and FAK supports cell division independent of the activity of the cell cycle-regulating CDK4/6 kinases.Oncogene · 2019Article
- Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks.Chemical reviews · 2018Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Mutational studies of human DNA helicase B (HDHB) have suggested that its activity is critical for the G1/S transition of the cell cycle, but the nature of its role remains unknown. In this study, we show that during G1, ectopically expressed HDHB localizes in nuclear foci induced by DNA damaging agents and that this focal pattern requires active HDHB. During S and G2/M, HDHB localizes primarily in the cytoplasm. A carboxy-terminal domain from HDHB confers cell cycle-dependent localization, but not the focal pattern, to a reporter protein. A cluster of potential cyclin-dependent kinase phosphorylation sites in this domain was modified at the G1/S transition and maintained through G2/M of the cell cycle in vivo, coincident with nuclear export of HDHB. Serine 967 of HDHB was the major site phosphorylated in vivo and in vitro by cyclin-dependent kinases. Mutational analysis demonstrated that phosphorylation of serine 967 is crucial in regulating the subcellular localization of ectopically expressed HDHB. We propose that the helicase of HDHB operates primarily during G1 to process endogenous DNA damage before the G1/S transition, and it is largely sequestered in the cytoplasm during S/G2.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.