ArticleThe Journal of physiology2004
Response of human cells to desiccation: comparison with hyperosmotic stress response.
Article in The Journal of physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 69 citations in OpenAlex.
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- Cytotoxic effect of protic ionic liquids in HepG2 and HaCat human cells:Toxicology research · 2019Article
- Article
- Chloroquine Protects Human Corneal Epithelial Cells from Desiccation Stress Induced Inflammation without Altering the Autophagy Flux.BioMed research international · 2018Article
- Cross-Study Comparison Reveals Common Genomic, Network, and Functional Signatures of Desiccation Resistance in Drosophila melanogaster.Molecular biology and evolution · 2016Article
- Osmotic stress-induced phosphorylation of H2AX by polo-like kinase 3 affects cell cycle progression in human corneal epithelial cells.The Journal of biological chemistry · 2014Article
- Decrease in hyperosmotic stress-induced corneal epithelial cell apoptosis by L-carnitine.Molecular vision · 2013Article
- The estrogen-related receptor alpha upregulates secretin expressions in response to hypertonicity and angiotensin II stimulation.PloS one · 2012Article
- Hyperosmotic stress-induced ATF-2 activation through Polo-like kinase 3 in human corneal epithelial cells.The Journal of biological chemistry · 2011Article
- Desiccation survival in an Antarctic nematode: molecular analysis using expressed sequenced tags.BMC genomics · 2009Article
- Hydrophilic protein associated with desiccation tolerance exhibits broad protein stabilization function.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Molecular defense mechanisms of Barrett's metaplasia estimated by an integrative genomics.Journal of molecular medicine (Berlin, Germany) · 2007Article
- Hyperosmotic stress response: comparison with other cellular stresses.Pflugers Archiv : European journal of physiology · 2007Review
- Mechanistic pathways and biological roles for receptor-independent activators of G-protein signaling.Pharmacology & therapeutics · 2007Review
- TRPV4 as osmosensor: a transgenic approach.Pflugers Archiv : European journal of physiology · 2005Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Increasing interest in anhydrobiosis ('life without water') has prompted the use of mammalian cells as a model in which candidate adaptations suspected of conferring desiccation tolerance can be tested. Despite this, there is no information on whether mammalian cells are able to sense and respond to desiccation. We have therefore examined the effect of desiccation on stress signalling pathways and on genes which are proposed to be expressed in response to water loss through osmotic stress. Depending on the severity of the drying regime, human cells survived for at least 24 h. Both SAPK/JNK and p38 mitogen-activated protein kinases (MAPKs) were activated within 30 min by desiccation as well as by all osmotica tested, and therefore MAPK pathways probably play an important role in both responses. Gene induction profiles differed under the two stress conditions, however: quantitative polymerase chain reaction (PCR) experiments showed that AR, BGT-1 and SMIT, which encode proteins governing organic osmolyte accumulation, were induced by hypersalinity but not by desiccation. This was surprising, since these genes have been proposed to be regulated by ionic strength and cell volume, both of which should be significantly affected in drying cells. Further investigation demonstrated that AR, BGT-1 and SMIT expression was dependent on the nature of the osmolyte. This suggests that their regulation involves factors other than intracellular ionic strength and cell volume changes, consistent with the lack of induction by desiccation. Our results show for the first time that human cells react rapidly to desiccation by MAPK activation, and that the response partially overlaps with that to hyperosmotic stress.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.