ArticleAnnals of botany2004
Phenylpropanoids, phenylalanine ammonia lyase and peroxidases in elicitor-challenged cassava (Manihot esculenta) suspension cells and leaves.
Article in Annals of botany, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 145 citations in OpenAlex.
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- Elicitation of theFrontiers in plant science · 2022Article
- PAL1 gene of the phenylpropanoid pathway increases resistance to the Cassava brown streak virus in cassava.Virology journal · 2021Article
- Identifying the Compounds of the Metabolic Elicitors ofPlants (Basel, Switzerland) · 2020Article
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- The Arabidopsis non-host defence-associated coumarin scopoletin protects soybean from Asian soybean rust.The Plant journal : for cell and molecular biology · 2019Article
- Cu-chitosan nanoparticle boost defense responses and plant growth in maize (Zea mays L.).Scientific reports · 2017Article
- Meristem Plant Cells as a Sustainable Source of Redox Actives for Skin Rejuvenation.Biomolecules · 2017Review
- Proteomics Coupled with Metabolite and Cell Wall Profiling Reveal Metabolic Processes of a Developing Rice Stem Internode.Frontiers in plant science · 2017Article
- Maize Fungal Growth Control with Scopoletin of Cassava Roots Produced in Benin.International journal of microbiology · 2017Article
- Abiotic elicitors mediated elicitation of innate immunity in tomato: an ex vivo comparison.Physiology and molecular biology of plants : an international journal of functional plant biology · 2016Article
- Comparative Proteomic Analysis Provides Insight into the Key Proteins Involved in Cucumber (Frontiers in plant science · 2016Article
- Phenylacetic Acid Is ISR Determinant Produced by Bacillus fortis IAGS162, Which Involves Extensive Re-modulation in Metabolomics of Tomato to Protect against Fusarium Wilt.Frontiers in plant science · 2016Article
- Differential gene expression in foxtail millet during incompatible interaction with Uromyces setariae-italicae.PloS one · 2015Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsControl of diseases in the key tropical staple, cassava, is dependent on resistant genotypes, but the innate mechanisms are unknown. The aim was to study phenylpropanoids and associated enzymes as possible defence components.
methodsPhenylalanine ammonia-lyase (PAL), phenylpropanoids and peroxidases (POD) were investigated in elicited cassava suspension cells and leaves. Yeast elicitor was the most effective of several microbial and endogenous elicitors. Fungitoxicity was determined against the cassava pathogens Fusarium solani, F. oxysporum and the saprotroph Trichoderma harzianum. KEY
resultsA single and rapid (> or =2-3 min) oxidative burst, measured as hydrogen peroxide, occurred in elicited cells. PAL activity was induced maximally at 15 h and was preceded by PAL mRNA accumulation, which peaked at 9 h. Symplasmic POD activity increased four-fold in cells, 48 h post-elicitation. POD isoforms (2-7 isoforms, pI 3.1-8.8) were detected in elicited and unelicited cells, extracellular medium and leaves but two extracellular isoforms were enhanced post-elicitation. Also expression of a cassava peroxidase gene MecPOD1 increased in elicited cells. Only anionic forms oxidized scopoletin, with highest activity by isoform pI 3.6, present in all samples. Unidentified phenolics and possibly scopolin increased post-elicitation, but there was no enhancement of scopoletin, rutin or kaempferol-3-O-rutinoside concentration. Fungal germ tube elongation was inhibited more than germination by esculetin, ferulic acid, quercetin and scopoletin. T. harzianum was generally more sensitive than the pathogens and was inhibited by > or =50 microg mL(-1) of ferulic acid and quercetin and > or =10 microg mL(-1) of scopoletin.
conclusionsPhenolic levels in cells were not enhanced and were, theoretically, too low to be inhibitory. However, in combination and when oxidized they may contribute to defence, because oxidation of esculetin and scopoletin by peroxidase and of esculetin by tyrosinase enhanced their fungitoxicity up to 20-fold.
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