ArticleJournal of the American Society for Mass Spectrometry2004
Development of an LC-MALDI method for the analysis of protein complexes.
Article in Journal of the American Society for Mass Spectrometry, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 77 citations in OpenAlex.
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- The Parkinson's disease kinase LRRK2 autophosphorylates its GTPase domain at multiple sites.Biochemical and biophysical research communications · 2009Article
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- Applying gene expression, proteomics and single-nucleotide polymorphism analysis for complex trait gene identification.Genetics · 2008Article
- A comparison of nLC-ESI-MS/MS and nLC-MALDI-MS/MS for GeLC-based protein identification and iTRAQ-based shotgun quantitative proteomics.Journal of biomolecular techniques : JBT · 2007Article
- Article
- Fragmentation of leucine enkephalin as a function of laser fluence in a MALDI TOF-TOF.Journal of the American Society for Mass Spectrometry · 2007Article
- Analytical model of peptide mass cluster centres with applications.Proteome science · 2006Article
- A phosphorylation map of the bovine papillomavirus E1 helicase.Virology journal · 2006Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, a two-dimensional LC-MALDI-TOF/TOF method has been developed for analyzing protein complexes. In our hands, the method has proven to be an excellent strategy for the analysis of protein complexes isolated in pull-down experiments. This is in part because the preservation of the chromatographic separation on a MALDI target yields an "unlimited" amount of time to obtain MS/MS spectra, making it possible to probe more deeply into complex samples. A brief statistical analysis was performed on the data obtained from the LC-MALDI-TOF/TOF system in order to better understand peptide fragmentation patterns under high-energy collision conditions. These statistical analyses provided some insight into how to evaluate the quality and accuracy of the database search results derived from the TOF/TOF-based analysis. The potential of the method was demonstrated by the successful identification of all the known penicillin-binding proteins in E. coli isolated using a drug-based pull-down with ampicillin as the bait. The performance of the LC-MALDI-TOF/TOF system was compared with that of an equivalent 2D LC-ESI-MS/MS approach, in the analysis of a protein bait-based pull-down. Regardless of the number of peptides identified in the ESI versus MALDI approach, the two approaches were found to be complementary. When the data is merged at the peptide level, the combined result gives higher Mascot scores and an overall higher confidence in protein identification than with either approach alone.
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