ArticleMolecular and cellular biology2004
Identification of a distinctive mutation spectrum associated with high levels of transcription in yeast.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Article
- Article
- Review
- Ribonucleotides and Transcription-Associated Mutagenesis in Yeast.Journal of molecular biology · 2017Review
- Genetic instability in budding and fission yeast-sources and mechanisms.FEMS microbiology reviews · 2015Review
- Transcription-associated mutagenesis.Annual review of genetics · 2014Review
- Article
- High levels of transcription stimulate transversions at GC base pairs in yeast.Environmental and molecular mutagenesis · 2013Article
- Role for topoisomerase 1 in transcription-associated mutagenesis in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Topoisomerase 1 provokes the formation of short deletions in repeated sequences upon high transcription in Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Article
- Transcription increases methylmethane sulfonate-induced mutations in alkB strains of Escherichia coli.DNA repair · 2008Article
- Article
- Activation-induced cytidine deaminase action is strongly stimulated by mutations of the THO complex.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Mutant LYS2 mRNAs retained and degraded in the nucleus of Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
High levels of transcription are associated with increased mutation rates in Saccharomyces cerevisiae, a phenomenon termed transcription-associated mutation (TAM). To obtain insight into the mechanism of TAM, we obtained LYS2 forward mutation spectra under low- versus high-transcription conditions in which LYS2 was expressed from either the low-level pLYS2 promoter or the strong pGAL1-10 promoter, respectively. Because of the large size of the LYS2 locus, forward mutations first were mapped to specific LYS2 subregions, and then those mutations that occurred within a defined 736-bp target region were sequenced. In the low-transcription strain base substitutions comprised the majority (64%) of mutations, whereas short insertion-deletion mutations predominated (56%) in the high-transcription strain. Most notably, deletions of 2 nucleotides (nt) comprised 21% of the mutations in the high-transcription strain, and these events occurred predominantly at 5'-(G/C)AAA-3' sites. No -2 events were present in the low-transcription spectrum, thus identifying 2-nt deletions as a unique mutational signature for TAM.
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