ArticleProceedings of the National Academy of Sciences of the United States of America2004
Establishment of a normal medakafish spermatogonial cell line capable of sperm production in vitro.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
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86 citing papers in PubMed.
- Article
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- Establishment and Characterization of a Long-Term Ovarian Cell Line (SBO) from Asian Seabass (International journal of molecular sciences · 2026Article
- Amhy/Amhr2y-mediated sex determination in two distantly related teleosts relies on the conserved Alk3-Smad5 axis.Molecular biology and evolution · 2026Article
- A single-cell multiomics roadmap of zebrafish spermatogenesis reveals regulatory principles of male germline formation.Molecular systems biology · 2026Article
- Non-CG DNA methylation in animal genomes.Nature genetics · 2025Review
- Establishment and Characteristics of the Spermatogonial Stem Cell Line from the Yellow River Carp (Biology · 2025Article
- Establishment, identification, and transcriptome analysis of a Sertoli cell line from ovoviviparous black rockfish Sebastes schlegelii.Fish physiology and biochemistry · 2025Article
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- Genomic Analysis and Functional Validation of Bidirectional Promoters in Medaka (International journal of molecular sciences · 2024Article
- Review
- Establishment and Characterization of a Spermatogonial Stem Cell Line from Tiger Puffer Fish (Animals : an open access journal from MDPI · 2023Article
- Establishment of aBiology · 2023Article
- Transcriptomes of Testes at Different Developmental Stages in the Opsariichthys bidens Predict Key Genes for Testis Development and Spermatogenesis.Marine biotechnology (New York, N.Y.) · 2023Article
- The Role of Promyelocytic Leukemia Zinc Finger (PLZF) and Glial-Derived Neurotrophic Factor Family Receptor Alpha 1 (GFRα1) in the Cryopreservation of Spermatogonia Stem Cells.International journal of molecular sciences · 2023Review
- Characterization of the In Vitro Cultured Ovarian Cells in the Asian Yellow Pond Turtle (Biology · 2022Article
- Establishment of a Spermatogonial Stem Cell Line with Potential of Meiosis in a Hermaphroditic Fish,Cells · 2022Article
26 more citing papers are in PubMed but not listed here.
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9 authors.
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Abstract
Spermatogonia are the male germ stem cells that continuously produce sperm for the next generation. Spermatogenesis is a complicated process that proceeds through mitotic phase of stem cell renewal and differentiation, meiotic phase, and postmeiotic phase of spermiogenesis. Full recapitulation of spermatogenesis in vitro has been impossible, as generation of normal spermatogonial stem cell lines without immortalization and production of motile sperm from these cells after long-term culture have not been achieved. Here we report the derivation of a normal spermatogonial cell line from a mature medakafish testis without immortalization. After 140 passages during 2 years of culture, this cell line retains stable but growth factor-dependent proliferation, a diploid karyotype, and the phenotype and gene expression pattern of spermatogonial stem cells. Furthermore, we show that this cell line can undergo meiosis and spermiogenesis to generate motile sperm. Therefore, the ability of continuous proliferation and sperm production in culture is an intrinsic property of medaka spermatogonial stem cells, and immortalization apparently is not necessary to derive male germ cell cultures. Our findings and cell line will offer a unique opportunity to study and recapitulate spermatogenesis in vitro and to develop approaches for germ-line transmission.
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