ArticleNucleic acids research2004
Localization and dynamics of small circular DNA in live mammalian nuclei.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Anticodon-edited transfer RNAs (ACE-tRNAs) encoded as therapeutic nonviral minimal DNA vectors.Nucleic acids research · 2026Article
- SMC5/6-mediated plasmid silencing is directed by SIMC1-SLF2 and antagonized by the SV40 large T antigen.eLife · 2025Article
- Anticodon Edited Transfer RNAs (ACE-tRNAs) Encoded as Therapeutic Nonviral Minimal DNA Vectors.bioRxiv : the preprint server for biology · 2025Article
- Live-Cell Imaging Shows Uneven Segregation of Extrachromosomal DNA Elements and Transcriptionally Active Extrachromosomal DNA Hubs in Cancer.Cancer discovery · 2022Article
- Evolution of the duplicated intracellular lipid-binding protein genes of teleost fishes.Molecular genetics and genomics : MGG · 2017Review
- Characterization of exogenous DNA mobility in live cells through fluctuation correlation spectroscopy.Scientific reports · 2015Article
- Tethered particle analysis of supercoiled circular DNA using peptide nucleic acid handles.Nature protocols · 2014Article
- Stable S/MAR-based episomal vectors are regulated at the chromatin level.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
- Persistent episomal transgene expression in liver following delivery of a scaffold/matrix attachment region containing non-viral vector.Gene therapy · 2008Article
- Similar active genes cluster in specialized transcription factories.The Journal of cell biology · 2008Article
- Intranuclear trafficking of episomal DNA is transcription-dependent.Molecular therapy : the journal of the American Society of Gene Therapy · 2007Article
- Tissue distribution of a plasmid DNA encoding Hsp65 gene is dependent on the dose administered through intramuscular delivery.Genetic vaccines and therapy · 2006Article
- Directional motion of foreign plasmid DNA to nuclear HP1 foci.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2006Article
- Tracking of microinjected DNA in live cells reveals the intracellular behavior and elimination of extrachromosomal genetic material.Nucleic acids research · 2005Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While genomic DNA, packaged into chromatin, is known to be locally constrained but highly dynamic in the nuclei of living cells, little is known about the localization and dynamics of small circular DNA molecules that invade cells by virus infection, application of gene therapy vectors or experimental transfection. To address this point, we have created traceable model substrates by direct labeling of plasmid DNA with fluorescent peptide nucleic acids, and have investigated their fate after microinjection into living cells. Here, we report that foreign DNA rapidly undergoes interactions with intranuclear structural sites that strongly reduce its mobility and restrict the DNA to regions excluding nucleoli and nuclear bodies such as PML bodies. The labeled plasmids partially co-localize with SAF-A, a well characterized marker protein for the nuclear 'scaffold' or 'matrix', and are resistant towards extraction by detergent and, in part, elevated salt concentrations. We show that the localization and the low mobility of plasmids is independent of the plasmid sequence, and does not require the presence of either a scaffold attachment region (SAR) DNA element or a functional promoter.
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