ArticleJournal of virology2004
Integrase-specific enhancement and suppression of retroviral DNA integration by compacted chromatin structure in vitro.
Article in Journal of virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 59 citations in OpenAlex.
- Brief Histories of Retroviral Integration Research and Associated International Conferences.Viruses · 2024Review
- Review
- HIV-1 Preintegration Complex Preferentially Integrates the Viral DNA into Nucleosomes Containing Trimethylated Histone 3-Lysine 36 Modification and Flanking Linker DNA.Journal of virology · 2022Article
- Integrating transposable elements in the 3D genome.Mobile DNA · 2020Article
- Physical principles of retroviral integration in the human genome.Nature communications · 2019Article
- Prototype foamy virus intasome aggregation is mediated by outer protein domains and prevented by protocatechuic acid.Scientific reports · 2019Article
- Interactions between Retroviruses and the Host Cell Genome.Molecular therapy. Methods & clinical development · 2018Review
- Integration site selection by retroviruses and transposable elements in eukaryotes.Nature reviews. Genetics · 2017Review
- Retroviral integrase protein and intasome nucleoprotein complex structures.World journal of biological chemistry · 2017Review
- DNA minicircles clarify the specific role of DNA structure on retroviral integration.Nucleic acids research · 2016Article
- Removal of nuclease contamination during purification of recombinant prototype foamy virus integrase.Journal of virological methods · 2016Article
- Sites of retroviral DNA integration: From basic research to clinical applications.Critical reviews in biochemistry and molecular biology · 2016Review
- Retroviral Integrase: Then and Now.Annual review of virology · 2015Review
- Key determinants of target DNA recognition by retroviral intasomes.Retrovirology · 2015Article
- Intasome architecture and chromatin density modulate retroviral integration into nucleosome.Retrovirology · 2015Article
- DNA Physical Properties and Nucleosome Positions Are Major Determinants of HIV-1 Integrase Selectivity.PloS one · 2015Article
- Retroviral DNA Transposition: Themes and Variations.Microbiology spectrum · 2014Article
- Preclinical profile of BI 224436, a novel HIV-1 non-catalytic-site integrase inhibitor.Antimicrobial agents and chemotherapy · 2014Article
- Understanding lentiviral vector chromatin targeting: working to reduce insertional mutagenic potential for gene therapy.Gene therapy · 2013Review
- Retroviral integrases promote fraying of viral DNA ends.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Integration of viral DNA into the host chromosome is an obligatory step in retroviral replication and is dependent on the activity of the viral enzyme integrase. To examine the influence of chromatin structure on retroviral DNA integration in vitro, we used a model target comprising a 13-nucleosome extended array that includes binding sites for specific transcription factors and can be compacted into a higher-ordered structure. We found that the efficiency of in vitro integration catalyzed by human immunodeficiency virus type 1 (HIV-1) integrase was decreased after compaction of this target with histone H1. In contrast, integration by avian sarcoma virus (ASV) integrase was more efficient after compaction by either histone H1 or a high salt concentration, suggesting that the compacted structure enhances this reaction. Furthermore, although site-specific binding of transcription factors HNF3 and GATA4 blocked ASV DNA integration in extended nucleosome arrays, local opening of H1-compacted chromatin by HNF3 had no detectable effect on integration, underscoring the preference of ASV for compacted chromatin. Our results indicate that chromatin structure affects integration site selection of the HIV-1 and ASV integrases in opposite ways. These distinct properties of integrases may also affect target site selection in vivo, resulting in an important bias against or in favor of integration into actively transcribed host DNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.