ArticleJournal of virology2004
Passive and active inclusion of host proteins in human immunodeficiency virus type 1 gag particles during budding at the plasma membrane.
Article in Journal of virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 39 citations in OpenAlex.
- Full assembly of HIV-1 particles requires assistance of the membrane curvature factor IRSp53.eLife · 2021Article
- Imaging extracellular vesicles: current and emerging methods.Journal of biomedical science · 2018Review
- Single-Particle Discrimination of Retroviruses from Extracellular Vesicles by Nanoscale Flow Cytometry.Scientific reports · 2017Article
- Biochemical and proteomic characterization of retrovirus Gag based microparticles carrying melanoma antigens.Scientific reports · 2016Article
- Formation and release of arrestin domain-containing protein 1-mediated microvesicles (ARMMs) at plasma membrane by recruitment of TSG101 protein.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Retroviral env glycoprotein trafficking and incorporation into virions.Molecular biology international · 2012Article
- Cellular kinases incorporated into HIV-1 particles: passive or active passengers?Retrovirology · 2011Review
- Clathrin facilitates the morphogenesis of retrovirus particles.PLoS pathogens · 2011Article
- Mechanisms for Env glycoprotein acquisition by retroviruses.AIDS research and human retroviruses · 2011Review
- Morphogenesis of the Infectious HIV-1 Virion.Frontiers in microbiology · 2011Article
- Axonal degeneration is blocked by nicotinamide mononucleotide adenylyltransferase (Nmnat) protein transduction into transected axons.The Journal of biological chemistry · 2010Article
- Host-encoded reporters for the detection and purification of multiple enveloped viruses.Journal of virological methods · 2010Article
- Purification of infectious human herpesvirus 6A virions and association of host cell proteins.Virology journal · 2007Article
- Host factors influencing susceptibility to HIV infection and AIDS progression.Retrovirology · 2007Review
- Packaging of actin into Ebola virus VLPs.Virology journal · 2005Article
- Analysis of human immunodeficiency virus type 1 Gag ubiquitination.Journal of virology · 2005Article
- Plunder and stowaways: incorporation of cellular proteins by enveloped viruses.Journal of virology · 2005Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human immunodeficiency virus type 1 particles form by budding at the surface of most cell types. In this process, a piece of the plasma membrane is modified into an enveloped virus particle. The process is driven by the internal viral protein Pr55(gag). We have studied how host proteins in the membrane are dealt with by Pr55(gag) during budding. Are they included in or excluded from the particle? The question was approached by measuring the relative concentrations of host and viral proteins in the envelope of Pr55(gag) particles and in their donor membranes in the cell. We observed that the bulk of the host proteins, including actin and clathrin, were passively included into the virus-like Gag particles. This result suggests that budding by Pr55(gag) proceeds without significant alteration of the original host protein composition at the cell membrane. Nevertheless, some proteins were concentrated in the particles, and a few were excluded. The concentrated proteins included cyclophilin A and Tsg-101. These were recruited to the plasma membrane by Pr55(gag). The membrane-bound cyclophilin A was concentrated into particles as efficiently as Pr55(gag), whereas Tsg-101 was concentrated more efficiently. The latter finding is consistent with a role for Tsg-101 in Gag particle release.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.