ArticleBMC developmental biology2004
Identification of Wnt responsive genes using a murine mammary epithelial cell line model system.
Article in BMC developmental biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Interstitial Fluid Shear Stress Induces the Synthetic Phenotype Switching of VSMCs to Release Pro-calcified Extracellular Vesicles via EGFR-MAPK-KLF5 Pathway.International journal of biological sciences · 2024Article
- ADAM10-Mediated Ectodomain Shedding Is an Essential Driver of Podocyte Damage.Journal of the American Society of Nephrology : JASN · 2021Article
- Overexpression of KLF5 is associated with poor survival and G1/S progression in pancreatic cancer.Aging · 2019Article
- The transcription factor Klf5 is essential for intrahepatic biliary epithelial tissue remodeling after cholestatic liver injury.The Journal of biological chemistry · 2018Article
- Dexamethasone induces docetaxel and cisplatin resistance partially through up-regulating Krüppel-like factor 5 in triple-negative breast cancer.Oncotarget · 2017Article
- Early Transcriptional Changes Induced by Wnt/Neural plasticity · 2016Article
- Recombinant R-spondin2 and Wnt3a up- and down-regulate novel target genes in C57MG mouse mammary epithelial cells.PloS one · 2012Article
- Integrated transcriptome and binding sites analysis implicates E2F in the regulation of self-renewal in human pluripotent stem cells.PloS one · 2011Article
- Article
- Kruppel-like factor 5 is not required for K-RasG12D lung tumorigenesis, but represses ABCG2 expression and is associated with better disease-specific survival.The American journal of pathology · 2010Article
- Regulation of the Rho family small GTPase Wrch-1/RhoU by C-terminal tyrosine phosphorylation requires Src.Molecular and cellular biology · 2010Article
- Essential role of KLF5 transcription factor in cell proliferation and differentiation and its implications for human diseases.Cellular and molecular life sciences : CMLS · 2009Review
- KLF5 promotes breast cell survival partially through fibroblast growth factor-binding protein 1-pERK-mediated dual specificity MKP-1 protein phosphorylation and stabilization.The Journal of biological chemistry · 2009Article
- Transcriptional profiling of putative human epithelial stem cells.BMC genomics · 2008Article
- Article
- Dynamic alterations in gene expression after Wnt-mediated induction of avian neural crest.Molecular biology of the cell · 2005Article
- Global gene expression profiling of cells overexpressing SMC3.Molecular cancer · 2005Article
- Ubiquitin-proteasome degradation of KLF5 transcription factor in cancer and untransformed epithelial cells.Oncogene · 2005Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
backgroundThe Wnt/Wg pathway plays an important role in the developmental program of many cells and tissues in a variety of organisms. In addition, many Wnts and components of their downstream signaling pathways, such as beta-catenin and APC, have been implicated in tumorigenesis. Over the past years, several genes have been identified as Wnt responsive, including c-myc, siamois, and cyclin D1.
resultsIn order to identify additional genes responsive to Wnt signaling that contribute to the transformed phenotype, we performed a cDNA subtractive hybridization screen between a mouse mammary epithelial cell line that overexpresses Wnt-1 (C57MG/Wnt-1) and the parental cell line (C57MG). The screen identified a total of 67 genes to be up-regulated in response to Wnt signaling. Of these 67 genes, the up-regulation of 62 was subsequently confirmed by Northern and dot blot analyses (and, for a subset, semi-quantitative PCR) of RNA isolated from C57MG cells subjected to (1) an independent Wnt-1 retroviral infection, and (2) co-culture with Wnt-1 expressing cells. Among the confirmed Wnt-1 responsive genes, we further characterized a mouse homolog of the human transcription factor Basic Transcription Element Binding protein 2 (BTEB2), Wnt-1 Responsive Cdc42 homolog (Wrch-1), and Wnt-1 Induced Secreted Protein (WISP-1).
conclusionSeveral novel genes were identified in this screen, as well as others that have been shown previously to be regulated by Wnt signaling, such as connexin43. The results indicate that cDNA subtractive hybridization is a useful method for identifying genes involved in the process of Wnt-1-induced transformation.
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