ArticlePharmaceutical research2004
In vivo phage display to identify M cell-targeting ligands.
Article in Pharmaceutical research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 53 citations in OpenAlex.
- Targeting the Gut Mucosal Immune System Using Nanomaterials.Pharmaceutics · 2021Review
- An in vivo selection-derived d-peptide for engineering erythrocyte-binding antigens that promote immune tolerance.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Mucus-penetrating phage-displayed peptides for improved transport across a mucus-like model.International journal of pharmaceutics · 2018Article
- Needle-Free Immunization with Chitosan-Based Systems.International journal of molecular sciences · 2018Review
- Peptides as drug delivery vehicles across biological barriers.Journal of pharmaceutical investigation · 2018Article
- pH-Responsive carriers for oral drug delivery: challenges and opportunities of current platforms.Drug delivery · 2017Review
- Ligand-directed profiling of organelles with internalizing phage libraries.Current protocols in protein science · 2015Article
- Bovine adenovirus-3 as a vaccine delivery vehicle.Vaccine · 2015Review
- Combinatorial peptide libraries: mining for cell-binding peptides.Chemical reviews · 2014Review
- Targeting mammalian organelles with internalizing phage (iPhage) libraries.Nature protocols · 2013Article
- M cell-targeted mucosal vaccine strategies.Current topics in microbiology and immunology · 2012Review
- pH-Responsive nanoparticles for drug delivery.Molecular pharmaceutics · 2010Review
- Ecological basis for rational phage therapy.Acta naturae · 2010Article
- Design opportunities for actively targeted nanoparticle vaccines.Nanomedicine (London, England) · 2008Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe purpose of this study was to use in vivo phage display screening technology to identify novel lead peptides that target delivery to M cells and to follicle-associated epithelium (FAE) of the intestine.
methodsPhage display libraries were screened in vivo within the gastrointestinal tract of a rat model by successive screenings across four cycles of selection.
resultsFollowing four cycles of in vivo screening, we identified 30 unique peptide sequences that bound to Peyer's patch tissue, human Caco-2, and rat IEC-6 epithelial cells. Two of the lead targeting peptides, peptides P8 (LETTCASLCYPS) and P25 (VPPHPMTYSCQY), were shown to bind to receptors on the surface of human intestinal tissue. The L-form, D-form, retro-inverted D-form, and selective Cys-to-Ala site-directed mutants of peptides P8 and P25 were also shown to retain binding to Caco-2 cell membranes when immobilized on the surface of a model particulate. Finally, the D-peptide analog of peptide P8 (yqcsytmphppv) enhanced the delivery of polystyrene particles to M cells in vivo in a mouse model, and these particles were delivered into Peyer's patch tissue, as determined by confocal microscopy.
conclusionsIn summary, we have identified novel ligands that target M cells and Peyer's patch tissue, and thus may have utility in the targeted oral delivery of vaccines and vaccine carrier systems to the mucosal immune system within the gastrointestinal tract.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.