ArticleMolecular genetics and genomics : MGG2004
Identification of genes preferentially expressed in the pathogenic yeast phase of Paracoccidioides brasiliensis, using suppression subtraction hybridization and differential macroarray analysis.
Article in Molecular genetics and genomics : MGG, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 69 citations in OpenAlex.
- One Century of Study: What We Learned aboutJournal of fungi (Basel, Switzerland) · 2021Review
- Cell Wall Synthesis, Development of Hyphae and Metabolic Pathways Are Processes Potentially Regulated by MicroRNAs Produced Between the Morphological Stages ofFrontiers in microbiology · 2018Article
- Histoplasma capsulatum surmounts obstacles to intracellular pathogenesis.The FEBS journal · 2016Review
- Construction and analysis of the cDNA subtraction library of yeast and mycelial phases of Sporothrix globosa isolated in China: identification of differentially expressed genes.Journal of Zhejiang University. Science. B · 2015Article
- Genome update of the dimorphic human pathogenic fungi causing paracoccidioidomycosis.PLoS neglected tropical diseases · 2014Article
- P. brasiliensis virulence is affected by SconC, the negative regulator of inorganic sulfur assimilation.PloS one · 2013Article
- Differentially expressed genes under simulated deep-sea conditions in the psychrotolerant yeast Cryptococcus sp. NIOCC#PY13.Extremophiles : life under extreme conditions · 2012Article
- Article
- Involvement of an alternative oxidase in oxidative stress and mycelium-to-yeast differentiation in Paracoccidioides brasiliensis.Eukaryotic cell · 2011Article
- Influence of 17β-estradiol on gene expression of Paracoccidioides during mycelia-to-yeast transition.PloS one · 2011Article
- Proteomic changes associated with deletion of the Magnaporthe oryzae conidial morphology-regulating gene COM1.Biology direct · 2010Article
- Genomic DNA microarray comparison of gene expression patterns in Paracoccidioides brasiliensis mycelia and yeasts in vitro.Microbiology (Reading, England) · 2009Article
- Mitochondrial function in the yeast form of the pathogenic fungus Paracoccidioides brasiliensis.Journal of bioenergetics and biomembranes · 2008Article
- Real-Time PCR: Revolutionizing Detection and Expression Analysis of Genes.Current genomics · 2007Article
- The transcriptome analysis of early morphogenesis in Paracoccidioides brasiliensis mycelium reveals novel and induced genes potentially associated to the dimorphic process.BMC microbiology · 2007Article
- Transcriptome analysis and molecular studies on sulfur metabolism in the human pathogenic fungus Paracoccidioides brasiliensis.Molecular genetics and genomics : MGG · 2006Article
- Transcriptome analysis of Aspergillus nidulans exposed to camptothecin-induced DNA damage.Eukaryotic cell · 2006Article
- Cell organisation, sulphur metabolism and ion transport-related genes are differentially expressed in Paracoccidioides brasiliensis mycelium and yeast cells.BMC genomics · 2006Article
- Article
- Real-time PCR in clinical microbiology: applications for routine laboratory testing.Clinical microbiology reviews · 2006Review
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Paracoccidioides brasiliensis, a thermodimorphic fungus, is the causative agent of paracoccidioidomycosis (PCM), the most prevalent systemic mycosis in Latin America. Pathogenicity appears to be intimately related to the dimorphic transition from the hyphal to the yeast form, which is induced by a shift from environmental temperature to the temperature of the mammalian host. Little information is available on the P. brasiliensis genes that are necessary during the pathogenic phase. We have therefore undertaken Suppression Subtraction Hybridization (SSH) and macroarray analyses with the aim of identifying genes that are preferentially expressed in the yeast phase. Genes identified by both procedures as being more highly expressed in the yeast phase are involved in basic metabolism, signal transduction, growth and morphogenesis, and sulfur metabolism. In order to test whether the observed changes in gene expression reflect the differences between the growth conditions used to obtain the two morphological forms rather than differences intrinsic to the cell types, we performed real-time RT-PCR experiments using RNAs derived from both yeast cells and mycelia that had been cultured at 37 degrees C and 26 degrees C in either complete medium (YPD or Sabouraud) or minimal medium. Twenty genes, including AGS1 (alpha-1,3-glucan synthase) and TSA1 (thiol-specific antioxidant), were shown to be more highly expressed in the yeast cells than in the hyphae. Although their levels of expression could be different in rich and minimal media, there was a general tendency for these genes to be more highly expressed in the yeast cells.
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