ArticlePlanta2004
A Zea mays 39-kDa thylakoid transglutaminase catalyses the modification by polyamines of light-harvesting complex II in a light-dependent way.
Article in Planta, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 64 citations in OpenAlex.
- At the core of global bioenergy: polyamines and transglutaminases in chloroplasts.Amino acids · 2026Review
- Review
- Polyamines - A New Metabolic Switch: Crosstalk With Networks Involving Senescence, Crop Improvement, and Mammalian Cancer Therapy.Frontiers in plant science · 2019Article
- TGase positively regulates photosynthesis via activation of Calvin cycle enzymes in tomato.Horticulture research · 2019Article
- Polyamines in Microalgae: Something Borrowed, Something New.Marine drugs · 2018Review
- Polyamine catabolism adds fuel to leaf senescence.Amino acids · 2017Review
- Endophytic Association ofMycobiology · 2016Article
- The Over-expression of the Plastidial Transglutaminase from Maize in Arabidopsis Increases the Activation Threshold of Photoprotection.Frontiers in plant science · 2016Article
- Genome Wide Association Mapping for the Tolerance to the Polyamine Oxidase Inhibitor Guazatine in Arabidopsis thaliana.Frontiers in plant science · 2016Article
- Dark-induced senescence of barley leaves involves activation of plastid transglutaminases.Amino acids · 2015Article
- Protective action of spermine and spermidine against photoinhibition of photosystem I in isolated thylakoid membranes.PloS one · 2014Article
- Senescence and programmed cell death in plants: polyamine action mediated by transglutaminase.Frontiers in plant science · 2014Review
- An unconventional road for the secretion of transglutaminase in pollen tubes?Plant signaling & behavior · 2013Article
- Regulation of Pollen Tube Growth by Transglutaminase.Plants (Basel, Switzerland) · 2013Review
- Role of plastid transglutaminase in LHCII polyamination and thylakoid electron and proton flow.PloS one · 2012Article
- Polyamines stimulate non-photochemical quenching of chlorophyll a fluorescence in Scenedesmus obliquus.Photosynthesis research · 2011Article
- Oxidative stress induced in tobacco leaves by chloroplast over-expression of maize plastidial transglutaminase.Planta · 2010Article
- RNA-seq analysis of sulfur-deprived Chlamydomonas cells reveals aspects of acclimation critical for cell survival.The Plant cell · 2010Article
- Transglutaminases and their substrates in kinetin-stimulated etioplast-to-chloroplast transformation in cucumber cotyledons.Protoplasma · 2008Article
- Transglutaminases: widespread cross-linking enzymes in plants.Annals of botany · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A transglutaminase (TGase; EC 2.3.2.13) activity, which shared many properties with the TGase activity of the Helianthus tuberosus chloroplast, was observed in the Zea mays L. chloroplast and in its fractions. This activity was found to be prevalent in thylakoids; bis-(glutamyl) spermidine and bis-(glutamyl) putrescine were the main polyamine conjugates formed. Light stimulated the endogenous thylakoid activity. Putrescine, spermidine and spermine were conjugated to the isolated light-harvesting complex of photosystem II (LHCII) with different degrees of efficiency, spermine being the polyamine most efficiently conjugated. A TGase with a light-sensitive activity was identified in the photosystem II-enriched fraction. Its partial purification on a sucrose gradient allowed the separation of a 39-kDa band, which was immunorecognised by two anti-TGase antibodies (Ab-3 and rat prostatic gland-TGase). Both a colorimetric and a radiometric assay for TGase activity, the former carried out in the presence of biotinylated cadaverine and the latter in the presence of polyamines labelled with radioactive isotopes and resulting in the isolation of glutamyl-polyamines, further confirmed that the thylakoid enzyme is indeed a calcium-dependent transglutaminase (Thyl-TGase). At variance with guinea pig liver and erythrocyte TGases, which are insensitive to light, the activity of the thylakoid transglutaminase is affected by light. Moreover, this enzyme, when tested with purified LHCII as substrate, catalysed the production of mono- and bis-glutamyl-polyamines in equal amounts, whereas the 'animal' enzymes produced mainly mono-derivatives. Herein, it is discussed whether this light sensitivity is due to the enzyme or the substrate.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.