ArticleThe Journal of membrane biology2004
Rate-limiting reactions determining different activation kinetics of Kv1.2 and Kv2.1 channels.
Article in The Journal of membrane biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- New High-Affinity Peptide Ligands for Kv1.2 Channel: Selective Blockers and Fluorescent Probes.Cells · 2024Article
- Mechanism of use-dependent Kv2 channel inhibition by RY785.The Journal of general physiology · 2022Article
- The AMIGO1 adhesion protein activates Kv2.1 voltage sensors.Biophysical journal · 2022Article
- High spatial density is associated with non-conducting Kv channels from two families.Biophysical journal · 2022Article
- EVAP: A two-photon imaging tool to study conformational changes in endogenous Kv2 channels in live tissues.The Journal of general physiology · 2021Article
- Kv2 channel-AMIGO β-subunit assembly modulates both channel function and cell adhesion molecule surface trafficking.Journal of cell science · 2021Article
- The tarantula toxin GxTx detains KThe Journal of general physiology · 2019Article
- Extracellular redox sensitivity of Kv1.2 potassium channels.Scientific reports · 2017Article
- Article
- Functional diversity of potassium channel voltage-sensing domains.Channels (Austin, Tex.) · 2016Review
- Voltage-dependent gating and gating charge measurements in the Kv1.2 potassium channel.The Journal of general physiology · 2015Article
- Uncoupling charge movement from channel opening in voltage-gated potassium channels by ruthenium complexes.The Journal of biological chemistry · 2011Article
- Position and role of the BK channel alpha subunit S0 helix inferred from disulfide crosslinking.The Journal of general physiology · 2008Article
- An activation gating switch in Kv1.2 is localized to a threonine residue in the S2-S3 linker.Biophysical journal · 2007Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To identify the mechanisms underlying the faster activation kinetics in Kv1.2 channels compared to Kv2.1 channels, ionic and gating currents were studied in rat Kv1.2 and human Kv2.1 channels heterologously expressed in mammalian cells. At all voltages the time course of the ionic currents could be described by an initial sigmoidal and a subsequent exponential component and both components were faster in Kv1.2 than in Kv2.1 channels. In Kv1.2 channels, the activation time course was more sigmoid at more depolarized potentials, whereas in Kv2.1 channels it was somewhat less sigmoid at more depolarized potentials. In contrast to the ionic currents, the ON gating currents were similarly fast for both channels. The main portion of the measured ON gating charge moved before the ionic currents were activated. The equivalent gating charge of Kv1.2 ionic currents was twice that of Kv2.1 ionic currents, whereas that of Kv1.2 ON gating currents was smaller than that of Kv2.1 ON gating currents. In conclusion, the different activation kinetics of Kv1.2 and Kv2.1 channels are caused by rate-limiting reactions that follow the charge movement recorded from the gating currents. In Kv1.2 channels, the reaction coupling the voltage-sensor movement to the pore opening contributes to rate limitation in a voltage-dependent fashion, whereas in Kv2.1 channels, activation is additionally rate-limited by a slow reaction in the subunit gating.
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