ArticleThe Journal of biological chemistry2004
TALE homeodomain proteins regulate gonadotropin-releasing hormone gene expression independently and via interactions with Oct-1.
Article in The Journal of biological chemistry, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 49 citations in OpenAlex.
- A single-nucleus multiomic study of preoptic area cells in prepubertal control vs prenatally androgenized female mice.Endocrinology · 2026Article
- Characterization of the human GnRH neuron developmental transcriptome using aDisease models & mechanisms · 2020Article
- Article
- New genetic regulators question relevance of abundant yolk protein production in C. elegans.Scientific reports · 2015Article
- Biochemistry of the tale transcription factors PREP, MEIS, and PBX in vertebrates.Developmental dynamics : an official publication of the American Association of Anatomists · 2014Review
- Meis: New friends of Pax.Neurogenesis (Austin, Tex.) · 2014Article
- Androgen receptor repression of gonadotropin-releasing hormone gene transcription via enhancer 1.Molecular and cellular endocrinology · 2012Article
- Androgen receptor repression of GnRH gene transcription.Molecular endocrinology (Baltimore, Md.) · 2012Article
- Dynamic chromatin modifications control GnRH gene expression during neuronal differentiation and protein kinase C signal transduction.Molecular endocrinology (Baltimore, Md.) · 2011Article
- Hypothalamic dysregulation and infertility in mice lacking the homeodomain protein Six6.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Unbiased, genome-wide in vivo mapping of transcriptional regulatory elements reveals sex differences in chromatin structure associated with sex-specific liver gene expression.Molecular and cellular biology · 2010Article
- Enhancers of GnRH transcription embedded in an upstream gene use homeodomain proteins to specify hypothalamic expression.Molecular endocrinology (Baltimore, Md.) · 2010Article
- Regulation of gonadotropin-releasing hormone-1 gene transcription by members of the purine-rich element-binding protein family.American journal of physiology. Endocrinology and metabolism · 2010Article
- Otx2 induction of the gonadotropin-releasing hormone promoter is modulated by direct interactions with Grg co-repressors.The Journal of biological chemistry · 2009Article
- Article
- Heterogeneous nuclear ribonucleoprotein A/B and G inhibits the transcription of gonadotropin-releasing-hormone 1.Molecular and cellular neurosciences · 2008Article
- p160 Myb-binding protein interacts with Prep1 and inhibits its transcriptional activity.Molecular and cellular biology · 2007Article
- Hormonal regulation of clonal, immortalized hypothalamic neurons expressing neuropeptides involved in reproduction and feeding.Molecular neurobiology · 2007Article
- Calcium influx and DREAM protein are required for GnRH gene expression pulse activity.Molecular and cellular endocrinology · 2007Article
- Persistent transactivation by meis1 replaces hox function in myeloid leukemogenesis models: evidence for co-occupancy of meis1-pbx and hox-pbx complexes on promoters of leukemia-associated genes.Molecular and cellular biology · 2006Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Gonadotropin-releasing hormone (GnRH) is the central regulator of reproductive function. Expression of the GnRH gene is confined to a rare population of neurons scattered throughout the hypothalamus. Restricted expression of the rat GnRH gene is driven by a multicomponent enhancer and an evolutionarily conserved promoter. Oct-1, a ubiquitous POU homeodomain transcription factor, was identified as an essential factor regulating GnRH transcription in the GT1-7 hypothalamic neuronal cell line. In this study, we conducted a two-hybrid interaction screen in yeast using a GT1-7 cDNA library to search for specific Oct-1 cofactors. Using this approach, we isolated Pbx1b, a TALE homeodomain transcription factor that specifically associates with Oct-1. We show that heterodimers containing Pbx/Prep1 or Pbx/Meis1 TALE homeodomain proteins bind to four functional elements within the GnRH regulatory region, each in close proximity to an Oct-1-binding site. Cotransfection experiments indicate that TALE proteins are essential for GnRH promoter activity in the GT1-7 cells. Moreover, Pbx1 and Oct-1, as well as Prep1 and Oct-1, form functional complexes that enhance GnRH gene expression. Finally, Pbx1 is expressed in GnRH neurons in embryonic as well as mature mice, suggesting that the associations between TALE homeodomain proteins and Oct-1 regulate neuron-specific expression of the GnRH gene in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.