ArticleGut2004
Lactic acid bacteria secrete metabolites retaining anti-inflammatory properties after intestinal transport.
Article in Gut, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 112 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
112 citing papers in PubMed, 1 synthesis or guideline pooled it, 303 citations in OpenAlex.
- Gut microbiome in serious mental illnesses: A systematic review and critical evaluation.Schizophrenia research · 2021Pooled it
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- Clinical trial: Lactobacillus plantarum 299v (DSM 9843) improves symptoms of irritable bowel syndrome.World journal of gastroenterology · 2012Trial
- Trial
- A non-hydrolyzed, fermented milk formula reduces digestive and respiratory events in infants at high risk of allergy.European journal of clinical nutrition · 2011Trial
- Multifunctional Zinc Glycyrrhizinate-Integrated Gel for Enhanced Probiotic Delivery and Alleviation of Acute and Spontaneous Colitis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Microbially derived glutathione from Eubacterium rectale alleviates oxidative stress and promotes intestinal epithelial recovery.Microbiome · 2026Article
- Exploring the characteristics of gut microbiome changes in lung cancer patients and healthy controls.Scientific reports · 2026Article
- Human milk and infant formula influence lactobacilli metabolism in a species-specific manner.NPJ science of food · 2026Article
- The Role of Lactic Acid Bacteria in Colon Cancer Mitigation: Insights into Mechanisms of Action.Advances in experimental medicine and biology · 2026Review
- Progress in interventions for vaginal microecology.Frontiers in cellular and infection microbiology · 2026Review
- Beyond breastfeeding: a One Health Decalogue for nurturing the infant microbiota.Frontiers in nutrition · 2026Review
- Dietary Synbiotic Attenuated the Intestinal Inflammation in Weaned Piglets Challenged withAnimals : an open access journal from MDPI · 2025Article
- The natural symbiotic bacterium Enterococcus faecalis LX10 drives Bombyx mori refractoriness to Nosema bombycis infection via the secretion of enterococcin.BMC microbiology · 2025Article
- Review
- Effect ofFood science & nutrition · 2024Article
- Article
- Activation of chloride channels and promotion of bowel movements by heat-killedBioscience of microbiota, food and health · 2024Article
- Review
- Tea Tree Oil Improves Energy Metabolism, Non-Specific Immunity, and Microbiota Diversity via the Intestine-Hepatopancreas Axis inAntioxidants (Basel, Switzerland) · 2023Article
52 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundProbiotic bacteria have a beneficial effect on intestinal inflammation. In this study, we have examined the effect of lactic acid and commensal Gram positive (+) bacteria conditioned media (CM) on tumour necrosis factor alpha (TNF-alpha) release and the mechanisms involved.
methodsLipopolysaccharide (LPS) induced TNF-alpha secretion by peripheral blood mononuclear cells or the THP-1 cell line was monitored in the presence or absence of bacteria CM obtained from two probiotic strains, Bifidobacterium breve (Bb) and Streptococcus thermophilus (St), and three commensal bacterial strains (Bifidobacterium bifidum, Ruminococcus gnavus, and unidentified Streptococcus). Bb and St bacteria CM were allowed to cross filter grown intestinal epithelial cell monolayers (HT29-19A) to assess intestinal transport of active bacterial products. These products were characterised and their effect on LPS binding to THP-1 cells and nuclear factor kappa B (NF kappa B) activation assessed.
resultsDose dependent inhibition of LPS induced TNF-alpha secretion was noted for both probiotic bacteria CM (64% and 71% inhibition for Bb and St, respectively) and to a lesser extent commensal bacteria CM (21-32% inhibition). Active products from Bb and St were resistant to digestive enzymes and had a molecular mass <3000 Da. Their inhibitory effect was preserved after transepithelial transport across intestinal cell monolayers, mainly in inflammatory conditions. LPS-FITC binding to THP-1 cells and NF kappa B activation were significantly inhibited by Bb and St CM.
conclusionB breve and S thermophilus release metabolites exerting an anti-TNF-alpha effect capable of crossing the intestinal barrier. Commensal bacteria also display a TNF-alpha inhibitory capacity but to a lesser extent. These results underline the beneficial effect of commensal bacteria in intestinal homeostasis and may explain the role of some probiotic bacteria in alleviating digestive inflammation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.