ArticleThe Biochemical journal2004
Mycobacterium tuberculosis DnaA initiator protein: purification and DNA-binding requirements.
Article in The Biochemical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Structures of DnaA domain I reveal a dimer conserved across Actinomycetes.Nucleic acids research · 2026Article
- Novel Populations ofMicroorganisms · 2024Article
- Mycobacterium tuberculosis oriC sequestration by MtrA response regulator.Molecular microbiology · 2015Article
- Article
- Phosphorylation of Mycobacterium tuberculosis ParB participates in regulating the ParABS chromosome segregation system.PloS one · 2015Article
- oriC-encoded instructions for the initiation of bacterial chromosome replication.Frontiers in microbiology · 2014Review
- Mechanisms for initiating cellular DNA replication.Annual review of biochemistry · 2013Review
- Localization of acidic phospholipid cardiolipin and DnaA in mycobacteria.Tuberculosis (Edinburgh, Scotland) · 2011Article
- Synchronous replication initiation in novel Mycobacterium tuberculosis dnaA cold-sensitive mutants.Molecular microbiology · 2009Article
- In-vitro helix opening of M. tuberculosis oriC by DnaA occurs at precise location and is inhibited by IciA like protein.PloS one · 2009Article
- SMC protein-dependent chromosome condensation during aerial hyphal development in Streptomyces.Journal of bacteriology · 2009Article
- Mechanism for the TtDnaA-Tt-oriC cooperative interaction at high temperature and duplex opening at an unusual AT-rich region in Thermoanaerobacter tengcongensis.Nucleic acids research · 2007Article
- Architecture of bacterial replication initiation complexes: orisomes from four unrelated bacteria.The Biochemical journal · 2005Article
- Molecular insights into replication initiation in a multipartite genome harboring bacterium Deinococcus radiodurans.The Journal of biological chemistryArticle
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Mycobacterium tuberculosis oriC (the origin of chromosomal replication) region contains 13 non-perfect DnaA boxes. The M. tuberculosis initiator protein, DnaA, was overexpressed in Escherichia coli as a soluble His-tagged fusion protein. The purified protein His6MtDnaA was investigated for its binding properties to DnaA boxes from the oriC region. Gel retardation demonstrated that the DnaA from M. tuberculosis requires two DnaA boxes for efficient binding. Electron microscopy as well as DNase I footprinting showed that the His6MtDnaA protein binds to four specific regions, which correspond to the location of 11 out of 13 previously identified DnaA boxes within the M. tuberculosis oriC. Probably, in M. tuberculosis, DnaA molecules by co-operative binding of numerous 'non-perfect' DnaA boxes assemble along the oriC region and subsequently form a massive nucleoprotein complex.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.