ArticleThe journal of gene medicine2004
Excision of Sleeping Beauty transposons: parameters and applications to gene therapy.
Article in The journal of gene medicine, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
44 citing papers in PubMed.
- A Naturally Active Spy Transposon Discovered from the Insect Genome of Colletes gigas as a Promising Novel Gene Transfer Tool.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Passer, a highly active transposon from a fish genome, as a potential new robust genetic manipulation tool.Nucleic acids research · 2023Article
- The Past, Present, and Future of Non-Viral CAR T Cells.Frontiers in immunology · 2022Review
- Jumping Ahead withViruses · 2021Review
- The Integration Preference of Sleeping Beauty at Non-TA Site Is Related to the Transposon End Sequences.Frontiers in genetics · 2021Article
- A single amino acid switch converts the Sleeping Beauty transposase into an efficient unidirectional excisionase with utility in stem cell reprogramming.Nucleic acids research · 2020Article
- Sleeping Beauty Mouse Models of Cancer: Microenvironmental Influences on Cancer Genetics.Frontiers in oncology · 2019Review
- Consider Changing the Horse for Your CAR-T?Molecular therapy : the journal of the American Society of Gene Therapy · 2018Article
- Preclinical and clinical advances in transposon-based gene therapy.Bioscience reports · 2017Review
- Retroviral vectors and transposons for stable gene therapy: advances, current challenges and perspectives.Journal of translational medicine · 2016Review
- piggyBac-ing models and new therapeutic strategies.Trends in biotechnology · 2015Review
- Transposon mutagenesis identifies genetic drivers of Braf(V600E) melanoma.Nature genetics · 2015Article
- Evaluating the potential for undesired genomic effects of the piggyBac transposon system in human cells.Nucleic acids research · 2015Article
- Sleeping Beauty transposon system for genetic etiological research and gene therapy of cancers.Cancer biology & therapy · 2015Review
- Non-viral generation of marmoset monkey iPS cells by a six-factor-in-one-vector approach.PloS one · 2015Article
- Sequencing methods and datasets to improve functional interpretation of sleeping beauty mutagenesis screens.BMC genomics · 2014Article
- The human application of gene therapy to re-program T-cell specificity using chimeric antigen receptors.Chinese journal of cancer · 2014Article
- Excision efficiency is not strongly coupled to transgenic rate: cell type-dependent transposition efficiency of sleeping beauty and piggyBac DNA transposons.Human gene therapy methods · 2014Article
- NMR structural analysis of Sleeping Beauty transposase binding to DNA.Protein science : a publication of the Protein Society · 2014Article
- Clinical applications of gamma delta T cells with multivalent immunity.Frontiers in immunology · 2014Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
A major problem in gene therapy is the determination of the rates at which gene transfer has occurred. Our work has focused on applications of the Sleeping Beauty (SB) transposon system as a non-viral vector for gene therapy. Excision of a transposon from a donor molecule and its integration into a cellular chromosome are catalyzed by SB transposase. In this study, we used a plasmid-based excision assay to study the excision step of transposition. We used the excision assay to evaluate the importance of various sequences that border the sites of excision inside and outside the transposon in order to determine the most active sequences for transposition from a donor plasmid. These findings together with our previous results in transposase binding to the terminal repeats suggest that the sequences in the transposon-junction of SB are involved in steps subsequent to DNA binding but before excision, and that they may have a role in transposase-transposon interaction. We found that SB transposons leave characteristically different footprints at excision sites in different cell types, suggesting that alternative repair machineries operate in concert with transposition. Most importantly, we found that the rates of excision correlate with the rates of transposition. We used this finding to assess transposition in livers of mice that were injected with the SB transposon and transposase. The excision assay appears to be a relatively quick and easy method to optimize protocols for delivery of genes in SB transposons to mammalian chromosomes in living animals.
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Registered trials
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