ArticlePlant physiology2004
Crop improvement through modification of the plant's own genome.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed.
- The evolving landscape of precise DNA insertion in plants.Nature communications · 2025Review
- Recent Advances in Antibiotic-Free Markers; Novel Technologies to Enhance Safe Human Food Production in the World.Molecular biotechnology · 2023Review
- Review
- Genetic Improvement of Wheat for Drought Tolerance: Progress, Challenges and Opportunities.Plants (Basel, Switzerland) · 2022Review
- The Intragenesis and Synthetic Biology Approach towards Accelerating Genetic Gains on Strawberry: Development of New Tools to Improve Fruit Quality and Resistance to Pathogens.Plants (Basel, Switzerland) · 2021Article
- An Insight into T-DNA Integration Events in Medicago sativa.International journal of molecular sciences · 2017Article
- Aldo-keto reductase enzymes detoxify glyphosate and improve herbicide resistance in plants.Plant biotechnology journal · 2017Article
- Advances in Maize Transformation Technologies and Development of Transgenic Maize.Frontiers in plant science · 2016Review
- Generation of Marker- and/or Backbone-Free Transgenic Wheat Plants viaFrontiers in plant science · 2016Article
- A bacterial gene codA encoding cytosine deaminase is an effective conditional negative selectable marker in Glycine max.Plant cell reports · 2015Article
- The codA gene as a negative selection marker in Citrus.SpringerPlus · 2015Article
- New biotechnology enhances the application of cisgenesis in plant breeding.Frontiers in plant science · 2014Review
- Less is more: strategies to remove marker genes from transgenic plants.BMC biotechnology · 2013Review
- Vector integration in triple R gene transformants and the clustered inheritance of resistance against potato late blight.Transgenic research · 2013Article
- Is genetic engineering ever going to take off in forage, turf and bioenergy crop breeding?Annals of botany · 2012Review
- Overexpression of the wild potato eIF4E-1 variant Eva1 elicits Potato virus Y resistance in plants silenced for native eIF4E-1.Transgenic research · 2012Article
- Utilization of PVX-Cre expression vector in potato.Transgenic research · 2012Article
- Recent advances in development of marker-free transgenic plants: regulation and biosafety concern.Journal of biosciences · 2012Review
- Enhanced production of single copy backbone-free transgenic plants in multiple crop species using binary vectors with a pRi replication origin in Agrobacterium tumefaciens.Transgenic research · 2011Article
- Backbone-free transformation of barrel medic (Medicago truncatula) with a Medicago-derived transfer DNA.Plant cell reports · 2010Article
Corrections and comments
- Retraction · 2013-04-01Error in Image · Error in Results and/or Conclusions · Unreliable Image · Unreliable Results and/or Conclusions ·
- Retracted
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant genetic engineering has, until now, relied on the incorporation of foreign DNA into plant genomes. Public concern about the extent to which transgenic crops differ from their traditionally bred counterparts has resulted in molecular strategies and gene choices that limit, but not eliminate, the introduction of foreign DNA. Here, we demonstrate that a plant-derived (P-) DNA fragment can be used to replace the universally employed Agrobacterium transfer (T-) DNA. Marker-free P-DNAs are transferred to plant cell nuclei together with conventional T-DNAs carrying a selectable marker gene. By subsequently linking a positive selection for temporary marker gene expression to a negative selection against marker gene integration, 29% of derived regeneration events contain P-DNA insertions but lack any copies of the T-DNA. Further refinements are accomplished by employing Omega-mutated virD2 and isopentenyl transferase cytokinin genes to impair T-DNA integration and select against backbone integration, respectively. The presented methods are used to produce hundreds of marker-free and backbone-free potato (Solanum tuberosum) plants displaying reduced expression of a tuber-specific polyphenol oxidase gene in potato. The modified plants represent the first example of genetically engineered plants that only contain native DNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.