ArticleMolecular biology of the cell2004
Use of fluorescent protein tags to study nuclear organization of the spliceosomal machinery in transiently transformed living plant cells.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
50 citing papers in PubMed, 90 citations in OpenAlex.
- Proteome profiling of plant Cajal bodies uncovers kingdom-specific components.Nucleic acids research · 2026Article
- At-RS31 orchestrates hierarchical cross-regulation of splicing factors and integrates alternative splicing with TOR-ABA pathways.The New phytologist · 2025Article
- FER-like iron deficiency-induced transcription factor (FIT) accumulates in nuclear condensates.The Journal of cell biology · 2024Article
- Detecting protein-protein interaction during liquid-liquid phase separation using fluorogenic protein sensors.Molecular biology of the cell · 2024Article
- Identification of a novel viral factor inducing tumorous symptoms by disturbing vascular developmentJournal of virology · 2023Article
- Completing the TRB family: newly characterized members show ancient evolutionary origins and distinct localization, yet similar interactions.Plant molecular biology · 2023Article
- Image analysis workflows to reveal the spatial organization of cell nuclei and chromosomes.Nucleus (Austin, Tex.) · 2022Article
- Function of Cajal Bodies in Nuclear RNA Retention inInternational journal of molecular sciences · 2022Article
- Crosstalk between H2A variant-specific modifications impacts vital cell functions.PLoS genetics · 2021Article
- An SMU Splicing Factor Complex Within Nuclear Speckles Contributes to Magnesium Homeostasis in Arabidopsis.Plant physiology · 2020Article
- Phase separation of Arabidopsis EMB1579 controls transcription, mRNA splicing, and development.PLoS biology · 2020Article
- Does co-transcriptional regulation of alternative splicing mediate plant stress responses?Nucleic acids research · 2019Review
- Thermo-Sensitive Alternative Splicing ofFrontiers in plant science · 2019Article
- The cyclin-dependent kinase G group defines a thermo-sensitive alternative splicing circuit modulating the expression of Arabidopsis ATU2AF65A.The Plant journal : for cell and molecular biology · 2018Article
- Subcellular Compartmentation of Alternatively Spliced Transcripts DefinesPlant physiology · 2018Article
- Active 5' splice sites regulate the biogenesis efficiency of Arabidopsis microRNAs derived from intron-containing genes.Nucleic acids research · 2017Article
- cDNA Library Screening Identifies Protein Interactors Potentially Involved in Non-Telomeric Roles of Arabidopsis Telomerase.Frontiers in plant science · 2015Article
- Rapid decline in nuclear costitutive photomorphogenesis1 abundance anticipates the stabilization of its target elongated hypocotyl5 in the light.Plant physiology · 2014Article
- Imaging of endogenous messenger RNA splice variants in living cells reveals nuclear retention of transcripts inaccessible to nonsense-mediated decay in Arabidopsis.The Plant cell · 2014Article
- Suitable transfection methods for single particle tracing in plant suspension cells.Plant methods · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although early studies suggested that little compartmentalization exists within the nucleus, more recent studies on metazoan systems have identified a still increasing number of specific subnuclear compartments. Some of these compartments are dynamic structures; indeed, protein and RNA-protein components can cycle between different domains. This is particularly evident for RNA processing components. In plants, lack of tools has hampered studies on nuclear compartmentalization and dynamics of RNA processing components. Here, we show that transient expression of fluorescent protein fusions of U1 and U2 small nuclear ribonucleoprotein particle (snRNP)-specific proteins U1-70K, U2B", and U2A ', nucleolar proteins Nop10 and PRH75, and serine-arginine-rich proteins in plant protoplasts results in their correct localization. Furthermore, snRNP-specific proteins also were correctly assembled into mature snRNPs. This system allowed a systematic analysis of the cellular localization of Arabidopsis serine-arginine-rich proteins, which, like their animal counterparts, localize to speckles but not to nucleoli and Cajal bodies. Finally, markers for three different nuclear compartments, namely, nucleoli, Cajal bodies, and speckles, have been established and were shown to be applicable for colocalization studies in living plant protoplasts. Thus, transient expression of proteins tagged with four different fluorescent proteins is a suitable system for studying the nuclear organization of spliceosomal proteins in living plant cells and should therefore allow studies of their dynamics as well.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.