ArticleMolecular biology of the cell2004
Exclusion of lipid rafts and decreased mobility of CD94/NKG2A receptors at the inhibitory NK cell synapse.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- Immunological synapse: structures, molecular mechanisms and therapeutic implications in disease.Signal transduction and targeted therapy · 2025Review
- All-Atom Simulations Reveal the Effect of Membrane Composition on the Signaling of the NKG2A/CD94/HLA-E Immune Receptor Complex.Journal of chemical information and modeling · 2024Article
- Assessment of membrane lipid state at the natural killer cell immunological synapse.Methods in cell biology · 2023Article
- Implications of NKG2A in immunity and immune-mediated diseases.Frontiers in immunology · 2022Review
- Degranulation enhances presynaptic membrane packing, which protects NK cells from perforin-mediated autolysis.PLoS biology · 2021Article
- Considerations of Antibody Geometric Constraints on NK Cell Antibody Dependent Cellular Cytotoxicity.Frontiers in immunology · 2020Review
- L-plastin is involved in NKG2D recruitment into lipid rafts and NKG2D-mediated NK cell migration.Journal of leukocyte biology · 2014Article
- Dynamics of natural killer cell receptor revealed by quantitative analysis of photoswitchable protein.Biophysical journal · 2013Article
- Role of lipid rafts in liver health and disease.World journal of gastroenterology · 2011Review
- Endocytosis and intracellular trafficking of human natural killer cell receptors.Traffic (Copenhagen, Denmark) · 2009Review
- Cryptococcus neoformans directly stimulates perforin production and rearms NK cells for enhanced anticryptococcal microbicidal activity.Infection and immunity · 2009Article
- Endocytosis as a mechanism of regulating natural killer cell function: unique endocytic and trafficking pathway for CD94/NKG2A.Immunologic research · 2009Review
- The killer's kiss: the many functions of NK cell immunological synapses.Current opinion in cell biology · 2008Review
- Formation and function of the lytic NK-cell immunological synapse.Nature reviews. Immunology · 2008Review
- NK cell survival mediated through the regulatory synapse with human DCs requires IL-15Ralpha.The Journal of clinical investigation · 2007Article
- Uncoupling of T-cell effector functions by inhibitory killer immunoglobulin-like receptors.Blood · 2006Article
- The human CD94 gene encodes multiple, expressible transcripts including a new partner of NKG2A/B.Genes and immunity · 2006Article
- The CD94/NKG2 family of receptors: from molecules and cells to clinical relevance.Immunologic research · 2006Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
CD94/NKG2A is an inhibitory receptor expressed by most human natural killer (NK) cells and a subset of T cells that recognizes human leukocyte antigen E (HLA-E) on potential target cells. To elucidate the cell surface dynamics of CD94/NKG2A receptors, we have expressed CD94/NKG2A-EGFP receptors in the rat basophilic leukemia (RBL) cell line. Photobleaching experiments revealed that CD94/NKG2A-EGFP receptors move freely within the plasma membrane and accumulate at the site of contact with ligand. The enriched CD94/NKG2A-EGFP is markedly less mobile than the nonligated receptor. We observed that not only are lipid rafts not required for receptor polarization, they are excluded from the site of receptor contact with the ligand. Furthermore, the lipid raft patches normally observed at the sites where FcepsilonR1 activation receptors are cross-linked were not observed when CD94/NKG2A was coengaged along with the activation receptor. These results suggest that immobilization of the CD94/NKG2A receptors at ligation sites not only promote sustenance of the inhibitory signal, but by lipid rafts exclusion prevent formation of activation signaling complexes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.