ArticleThe EMBO journal2004
Topography of the ISW2-nucleosome complex: insights into nucleosome spacing and chromatin remodeling.
Article in The EMBO journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
82 citing papers in PubMed, 142 citations in OpenAlex.
- Chromatin Remodelling Complexes in Plant Adaptation to Abiotic Stress: Mechanisms and Perspectives.Plant, cell & environment · 2026Review
- The ISW1 and CHD1 chromatin remodelers suppress global nucleosome dynamics in living yeast cells.Science advances · 2025Article
- Whole genome regulatory effect ofmBio · 2024Article
- Functionalized graphene-oxide grids enable high-resolution cryo-EM structures of the SNF2h-nucleosome complex without crosslinking.Nature communications · 2024Article
- Article
- ISWI catalyzes nucleosome sliding in condensed nucleosome arrays.bioRxiv : the preprint server for biology · 2023Article
- Nucleosome Core Particle Reconstitution with Recombinant Histones and Widom 601 DNA.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Structure and Function of Chromatin Remodelers.Journal of molecular biology · 2021Review
- Article
- Reconstitution and Purification of Nucleosomes with Recombinant Histones and Purified DNA.Current protocols in molecular biology · 2020Article
- Yeast chromatin remodeling complexes and their roles in transcription.Current genetics · 2020Review
- CHD4 slides nucleosomes by decoupling entry- and exit-side DNA translocation.Nature communications · 2020Article
- Cryo-EM structures of remodeler-nucleosome intermediates suggest allosteric control through the nucleosome.eLife · 2019Article
- Article
- Mechanisms of action and regulation of ATP-dependent chromatin-remodelling complexes.Nature reviews. Molecular cell biology · 2017Review
- Structural reorganization of the chromatin remodeling enzyme Chd1 upon engagement with nucleosomes.eLife · 2017Article
- Interdomain Communication of the Chd1 Chromatin Remodeler across the DNA Gyres of the Nucleosome.Molecular cell · 2017Article
- Distortion of histone octamer core promotes nucleosome mobilization by a chromatin remodeler.Science (New York, N.Y.) · 2017Article
- Article
- Genome-wide analysis reveals positional-nucleosome-oriented binding pattern of pioneer factor FOXA1.Nucleic acids research · 2016Article
22 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Linker DNA was found to be critical for the specific docking of ISW2 with nucleosomes as shown by mapping the physical contacts of ISW2 with nucleosomes at base-pair resolution. Hydroxyl radical footprinting revealed that ISW2 not only extensively interacts with the linker DNA, but also approaches the nucleosome from the side perpendicular to the axis of the DNA superhelix and contacts two disparate sites on the nucleosomal DNA from opposite sides of the superhelix. The topography of the ISW2-nucleosome was further delineated by finding which of the ISW2 subunits are proximal to specific sites within the linker and nucleosomal DNA regions by site-directed DNA photoaffinity labeling. Although ISW2 was shown to contact approximately 63 bp of linker DNA, a minimum of 20 bp of linker DNA was required for stable binding of ISW2 to nucleosomes. The remaining approximately 43 bp of flanking linker DNA promoted more efficient binding under competitive binding conditions and was functionally important for enhanced sliding of nucleosomes when ISW2 was significantly limiting.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.