ArticleJournal of clinical microbiology2004
Presence of bacterial phage-like DNA sequences in commercial Taq DNA polymerase reagents.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- DNA sequence contamination analyzer (DNASCAN): a supervised analysis toolkit for detecting and removing DNA contaminants.BMC bioinformatics · 2026Article
- CleanSeqU algorithm for decontamination of catheterized urine 16S rRNA sequencing data.Scientific reports · 2025Article
- The relationship between the gastric cancer microbiome and clinicopathological factors: a metagenomic investigation from the 100,000 genomes project and The Cancer Genome Atlas.Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association · 2025Article
- Bacterial and fungal characterization of pancreatic adenocarcinoma from Endoscopic Ultrasound-guided biopsies.Frontiers in immunology · 2023Article
- Elimination of "kitome" and "splashome" contamination results in lack of detection of a unique placental microbiome.BMC microbiology · 2020Article
- Detecting the presence of bacterial RNA by polymerase chain reaction in low volumes of preoperatively aspirated synovial fluid from prosthetic joint infections.Bone & joint research · 2020Article
- Application of next generation sequencing technology on contamination monitoring in microbiology laboratory.Biosafety and health · 2019Article
- Plasmid DNA contaminant in molecular reagents.Scientific reports · 2019Article
- Detecting the Presence of Bacterial DNA and RNA by Polymerase Chain Reaction to Diagnose Suspected Periprosthetic Joint Infection after Antibiotic Therapy.Orthopaedic surgery · 2018Article
- Limited Correlation of Shotgun Metagenomics Following Host Depletion and Routine Diagnostics for Viruses and Bacteria in Low Concentrated Surrogate and Clinical Samples.Frontiers in cellular and infection microbiology · 2018Article
- Analysis of the cervical microbiome and potential biomarkers from postpartum HIV-positive women displaying cervical intraepithelial lesions.Scientific reports · 2017Article
- Evaluation of Methods for the Concentration and Extraction of Viruses from Sewage in the Context of Metagenomic Sequencing.PloS one · 2017Article
- PEPR: pipelines for evaluating prokaryotic references.Analytical and bioanalytical chemistry · 2016Article
- Reagent and laboratory contamination can critically impact sequence-based microbiome analyses.BMC biology · 2014Article
- Escherichia coli XL10-gold bacteria produce bacteriophage.Journal of clinical microbiology · 2013Article
- Molecular diagnosis of periprosthetic joint infection by quantitative RT-PCR of bacterial 16S ribosomal RNA.TheScientificWorldJournal · 2013Article
- Next-generation sequencing in the analysis of human microbiota: essential considerations for clinical application.Molecular diagnosis & therapy · 2010Review
- An efficient multistrategy DNA decontamination procedure of PCR reagents for hypersensitive PCR applications.PloS one · 2010Article
- Article
- Presence of beta-lactamase gene TEM-1 DNA sequence in commercial Taq DNA polymerase.Journal of clinical microbiology · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many studies have reported the presence of bacterial DNA contamination in commercial Taq DNA polymerase reagents. This is the first report of the presence of phage-like DNA sequences in certain commercial Taq DNA polymerase reagents. Precautions are needed when using amplification reagents with exogenous DNAs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.