ArticleJournal of clinical microbiology2004
DNA microarray format for detection and subtyping of human papillomavirus.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 72 citations in OpenAlex.
- Prevalence of human papillomavirus and subtype distribution in male partners of women with cervical intraepithelial neoplasia (CIN): a systematic review.BMC infectious diseases · 2019Pooled it
- Discrimination of human papillomavirus genotypes using innovative technique nested-high resolution melting.Scientific reports · 2022Article
- Mucosal Alpha-Papillomavirus (HPV89) in a rare skin lesion.Virology journal · 2015Article
- A low density microarray method for the identification of human papillomavirus type 18 variants.Sensors (Basel, Switzerland) · 2013Article
- Use of consensus sequences for the design of high density resequencing microarrays: the influenza virus paradigm.BMC genomics · 2010Article
- Comparative analysis of four methods to extract DNA from paraffin-embedded tissues: effect on downstream molecular applications.BMC research notes · 2010Article
- Peptide nucleic acid-based array for detecting and genotyping human papillomaviruses.Journal of clinical microbiology · 2009Article
- Human papillomavirus genotyping by multiplex pyrosequencing in cervical cancer patients from India.Journal of biosciences · 2008Article
- PathogenMip assay: a multiplex pathogen detection assay.PloS one · 2007Article
- An in vitro selection scheme for oligonucleotide probes to discriminate between closely related DNA sequences.Nucleic acids research · 2007Article
- Oligonucleotide microarray with RD-PCR labeling technique for detection and typing of human papillomavirus.Current microbiology · 2006Article
- E-Predict: a computational strategy for species identification based on observed DNA microarray hybridization patterns.Genome biology · 2005Article
- Sentinel-base DNA genotyping using multiple sequencing primers for high-risk human papillomaviruses.Molecular and cellular probesArticle
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new human papillomavirus (HPV) assay using high-density DNA microarrays is described. An HPV DNA fragment from the 3' end of the E1 gene was amplified and digoxigenin labeled by PCR, and the resulting amplicons were hybridized onto type-specific oligonucleotides immobilized on high-density DNA microarrays. For detection, a simple immunohistochemical staining procedure was used with a substrate that has both colorimetric and fluorescent properties. This detection chemistry enables the rapid identification of reactive spots by regular light microscopy and semiquantification by laser scanning. Both single and multiple HPV infections are recognized by this assay, and the corresponding HPV types are easily identified. With this assay, 53 mucosal HPV types were detected and identified. A total of 45 HPV types were identified by a single type-specific probe, whereas the remaining 8 mucosal HPV types could be identified by a specific combination of probes. The simple assay format allows usage of this assay without expensive equipment, making it accessible to all diagnostic laboratories with PCR facilities.
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