ArticleJournal of clinical microbiology2004
Simultaneous analysis of multiple staphylococcal enterotoxin genes by an oligonucleotide microarray assay.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 96 citations in OpenAlex.
- Foodborne Pathogen Prevalence and Biomarker Identification for Microbial Contamination in Mutton Meat.Biology · 2024Article
- Coagulase-negative staphylococci are the main causes of bacterial meningitis in duck.Poultry science · 2024Article
- Molecular characterization of enterotoxin genes in methicillin-resistant S. aureus isolated from food poisoning outbreaks in Egypt.Journal of health, population, and nutrition · 2023Article
- Article
- Aerosol jet printing of biological inks by ultrasonic delivery.Biofabrication · 2020Article
- Safety Evaluation of the Coagulase-Negative Staphylococci Microbiota of Salami: Superantigenic Toxin Production and Antimicrobial Resistance.BioMed research international · 2015Article
- Microarray-based gene expression profiling to elucidate effectiveness of fermented Codonopsis lanceolata in mice.International journal of molecular sciences · 2014Article
- Characterization of Staphylococcus aureus strains associated with food poisoning in Shenzhen, China.Applied and environmental microbiology · 2012Article
- Genetically divergent methicillin-resistant Staphylococcus aureus and sec-dependent mastitis of dairy goats in Taiwan.BMC veterinary research · 2012Article
- Diverse enterotoxin gene profiles among clonal complexes of Staphylococcus aureus isolates from the Bronx, New York.Applied and environmental microbiology · 2009Article
- Rapid DNA amplification using a battery-powered thin-film resistive thermocycler.Methods in molecular biology (Clifton, N.J.) · 2009Article
- Carbon nanotubes with enhanced chemiluminescence immunoassay for CCD-based detection of Staphylococcal enterotoxin B in food.Analytical chemistry · 2008Article
- Plastic polymers for efficient DNA microarray hybridization: application to microbiological diagnostics.Journal of clinical microbiology · 2008Article
- Food microbial pathogen detection and analysis using DNA microarray technologies.Foodborne pathogens and disease · 2008Article
- Report of a strain of Staphylococcus caprae with the genes for enterotoxin A and enterotoxin-like toxin type P.Journal of clinical microbiology · 2007Article
- Biotyping of enterotoxigenic Staphylococcus aureus by enterotoxin gene cluster (egc) polymorphism and spa typing analyses.Applied and environmental microbiology · 2006Article
- The use of bacteriophages in eliminating polyresistant strains of Staphylococcus aureus and Streptococcus agalactiae.Folia microbiologica · 2005Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Staphylococcal enterotoxins (SEs) are a family of 17 major serological types of heat-stable enterotoxins that are one of the leading causes of gastroenteritis resulting from consumption of contaminated food. SEs are considered potential bioweapons. Many Staphylococcus aureus isolates contain multiple SEs. Because of the large number of SEs, serological typing and PCR typing are laborious and time-consuming. Furthermore, serological typing may not always be practical because of antigenic similarities among enterotoxins. We report on a microarray-based one-tube assay for the simultaneous detection and identification (genetic typing) of multiple enterotoxin (ent) genes. The proposed typing method is based on PCR amplification of the target region of the ent genes with degenerate primers, followed by characterization of the PCR products by microchip hybridization with oligonucleotide probes specific for each ent gene. We verified the performance of this method by using several other techniques, including PCR amplification with gene-specific primers, followed by gel electrophoresis or microarray hybridization, and sequencing of the enterotoxin genes. The assay was evaluated by analysis of previously characterized staphylococcal isolates containing 16 ent genes. The microarray assay revealed that some of these isolates contained additional previously undetected ent genes. The use of degenerate primers allows the simultaneous amplification and identification of as many as nine different ent genes in one S. aureus strain. The results of this study demonstrate the usefulness of the oligonucleotide microarray assay for the analysis of multitoxigenic strains, which are common among S. aureus strains, and for the analysis of microbial pathogens in general.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.