ArticleJournal of clinical microbiology2004
Comparison of conventional and molecular methods for identification of aerobic catalase-negative gram-positive cocci in the clinical laboratory.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it, 126 citations in OpenAlex.
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- Optimization of 16S RNA Sequencing and Evaluation of Metagenomic Analysis with Kraken 2 and KrakenUniq.Diagnostics (Basel, Switzerland) · 2025Article
- Identification and Safety Assessment ofMicroorganisms · 2024Article
- Comparison of an automated DNA extraction and 16S rDNA real time PCR/sequencing diagnostic method using optimized reagents with culture during a 15-month study using specimens from sterile body sites.BMC microbiology · 2022Article
- Periodontitis increases risk of viable bacteria in freshly drawn blood donations.Blood transfusion = Trasfusione del sangue · 2021Article
- Community structures and comparison of nosZ and 16S rRNA genes from culturable denitrifying bacteria.Folia microbiologica · 2020Article
- The Enterococcus: a Model of Adaptability to Its Environment.Clinical microbiology reviews · 2019Review
- Increased Pathogen Identification in Vascular Graft Infections by the Combined Use of Tissue Cultures and 16S rRNA Gene Polymerase Chain Reaction.Frontiers in medicine · 2018Article
- Whole genome sequencing as a tool for phylogenetic analysis of clinical strains of Mitis group streptococci.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016Article
- Direct Identification of Aerobic Bacteria by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry Is Accurate and Robust.Journal of clinical laboratory analysis · 2016Article
- Matrix-assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry (MALDI-TOF MS) as a Reliable Tool to Identify Species of Catalase-negative Gram-positive Cocci not Belonging to theThe open microbiology journal · 2016Article
- Molecular characterization of extended-spectrum beta-lactamases (ESBLs) produced by clinical isolates of Acinetobacter baumannii in Saudi Arabia.Annals of clinical microbiology and antimicrobials · 2015Article
- Article
- Molecular and Phenotypic Characterization of Aerococcus viridans Associated with Subclinical Bovine Mastitis.PloS one · 2015Article
- Viable bacteria associated with red blood cells and plasma in freshly drawn blood donations.PloS one · 2015Article
- The rebirth of culture in microbiology through the example of culturomics to study human gut microbiota.Clinical microbiology reviews · 2015Article
- Review
- Biofilm formation, gel and esp gene carriage among recreational beach Enterococci.Global journal of health science · 2014Article
- Sternal Osteomyelitis Caused by Gordonia bronchialis after Open-Heart Surgery.Infection & chemotherapy · 2014Article
- Accurate identification of fastidious Gram-negative rods: integration of both conventional phenotypic methods and 16S rRNA gene analysis.BMC microbiology · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Over a period of 18 months we have evaluated the use of 16S ribosomal DNA (rDNA) sequence analysis as a means of identifying aerobic catalase-negative gram-positive cocci in the clinical laboratory. A total of 171 clinically relevant strains were studied. The results of molecular analyses were compared with those obtained with a commercially available phenotypic identification system (API 20 Strep system; bioMérieux sa, Marcy l'Etoile, France). Phenotypic characterization identified 67 (39%) isolates to the species level and 32 (19%) to the genus level. Seventy-two (42%) isolates could not be discriminated at any taxonomic level. In comparison, 16S rDNA sequencing identified 138 (81%) isolates to the species level and 33 (19%) to the genus level. For 42 of 67 isolates assigned to a species with the API 20 Strep system, molecular analyses yielded discrepant results. Upon further analysis it was concluded that among the 42 isolates with discrepant results, 16S rDNA sequencing was correct for 32 isolates, the phenotypic identification was correct for 2 isolates, and the results for 8 isolates remained unresolved. We conclude that 16S rDNA sequencing is an effective means for the identification of aerobic catalase-negative gram-positive cocci. With the exception of Streptococcus pneumoniae and beta-hemolytic streptococci, we propose the use of 16S rDNA sequence analysis if adequate species identification is of concern.
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