ArticleJournal of clinical microbiology2004
Sequencing and comparative analysis of flagellin genes fliC, fljB, and flpA from Salmonella.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
49 citing papers in PubMed, 131 citations in OpenAlex.
- Assessing Salmonella Typhi Pathogenicity and Prevention: The Crucial Role of Vaccination in Combating Typhoid Fever.International journal of molecular sciences · 2025Review
- Phylogeny and divergence of the 100 most commonFrontiers in microbiology · 2025Article
- Bridging Classical Methodologies inMicroorganisms · 2024Review
- Development and Application of Real-Time PCR Assay for Detection of Salmonella Abortusequi.Journal of clinical microbiology · 2023Article
- Comparison of Phenotype and Genotype Virulence and Antimicrobial Factors ofInternational journal of molecular sciences · 2023Article
- Duplicated Flagellins in Pseudomonas Divergently Contribute to Motility and Plant Immune Elicitation.Microbiology spectrum · 2023Article
- The Prolonged Treatment ofInternational journal of molecular sciences · 2023Article
- Article
- Multiplex ligation reaction based on probe melting curve analysis: a pragmatic approach for the identification of 30 common Salmonella serovars.Annals of clinical microbiology and antimicrobials · 2019Article
- Prevalence and molecular characterization of Salmonella enterica serovar Typhimurium from ice and beverages in Jakarta, Indonesia.BMC research notes · 2019Article
- Embracing Diversity: Differences in Virulence Mechanisms, Disease Severity, and Host Adaptations Contribute to the Success of NontyphoidalFrontiers in microbiology · 2019Review
- Prevalence of Antimicrobial Resistance and Virulence Gene Elements ofFrontiers in microbiology · 2019Article
- Assessment and Comparison of Molecular Subtyping and Characterization Methods forFrontiers in microbiology · 2019Review
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- Comprehensive Analysis Reveals Two Distinct Evolution Patterns ofFrontiers in microbiology · 2017Article
- The Validation and Implications of Using Whole Genome Sequencing as a Replacement for Traditional Serotyping for a NationalFrontiers in microbiology · 2017Article
- Outer membrane vesicles from flagellin-deficient Salmonella enterica serovar Typhimurium induce cross-reactive immunity and provide cross-protection against heterologous Salmonella challenge.Scientific reports · 2016Article
- Article
- The O-Antigen Capsule of Salmonella enterica Serovar Typhimurium Facilitates Serum Resistance and Surface Expression of FliC.Infection and immunity · 2015Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Salmonella isolates have traditionally been classified by serotyping, the serologic identification of two surface antigens, O-polysaccharide and flagellin protein. Serotyping has been of great value in understanding the epidemiology of Salmonella and investigating disease outbreaks; however, production and quality control of the hundreds of antisera required for serotyping is difficult and time-consuming. To circumvent the problems associated with antiserum production, we began the development of a system for determination of serotype in Salmonella based on DNA markers. To identify flagellar antigen-specific sequences, we sequenced 280 alleles of the three genes that are known to encode flagellin in Salmonella, fliC, fljB, and flpA, representing 67 flagellar antigen types. Analysis of the data indicated that the sequences from fliC, fljB, and flpA clustered by the antigen(s) they encode not by locus. The sequences grouped into four clusters based on their conserved regions. Three of the four clusters included multiple flagellar antigen types and were designated the G complex, the Z4 complex, and the alpha cluster. The fourth cluster contained a single antigen type, H:z(29). The amino acid sequences of the conserved regions within each cluster have greater than 95% amino acid identity, whereas the conserved regions differ substantially between clusters (75 to 85% identity). Substantial sequence heterogeneity existed between alleles encoding different flagellar antigens while alleles encoding the same flagellar antigen were homologous, suggesting that flagellin genes may be useful targets for the molecular determination of flagellar antigen type.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.