ArticleMolecular microbiology2004
Branching sites and morphological abnormalities behave as ectopic poles in shape-defective Escherichia coli.
Article in Molecular microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed, 54 citations in OpenAlex.
- RdsA Is a Global Regulator That Controls Cell Shape and Division inFrontiers in microbiology · 2022Article
- Essential dynamic interdependence of FtsZ and SepF for Z-ring and septum formation in Corynebacterium glutamicum.Nature communications · 2020Article
- Swarmer Cell Development of the Bacterium Proteus mirabilis Requires the Conserved Enterobacterial Common Antigen Biosynthesis GeneJournal of bacteriology · 2018Article
- Substitution of Alanine at Position 184 with Glutamic Acid in Escherichia coli PBP5 Ω-Like Loop Introduces a Moderate Cephalosporinase Activity.The protein journal · 2018Article
- Article
- PBP deletion mutants of Escherichia coli exhibit irregular distribution of MreB at the deformed zones.Current microbiology · 2014Article
- Bacterial solutions to multicellularity: a tale of biofilms, filaments and fruiting bodies.Nature reviews. Microbiology · 2014Review
- The Rcs stress response and accessory envelope proteins are required for de novo generation of cell shape in Escherichia coli.Journal of bacteriology · 2013Article
- Characterization of osmotically induced filaments of Salmonella enterica.Applied and environmental microbiology · 2012Article
- Measuring the stiffness of bacterial cells from growth rates in hydrogels of tunable elasticity.Molecular microbiology · 2012Article
- Escherichia coli low-molecular-weight penicillin-binding proteins help orient septal FtsZ, and their absence leads to asymmetric cell division and branching.Molecular microbiology · 2012Article
- Sub-inhibitory cefsulodin sensitization of E. coli to β-lactams is mediated by PBP1b inhibition.PloS one · 2012Article
- Article
- A genome-scale proteomic screen identifies a role for DnaK in chaperoning of polar autotransporters in Shigella.Journal of bacteriology · 2009Article
- Sculpting the bacterial cell.Current biology : CB · 2009Review
- The peptidoglycan sacculus of Myxococcus xanthus has unusual structural features and is degraded during glycerol-induced myxospore development.Journal of bacteriology · 2009Article
- Cell shape and cell-wall organization in Gram-negative bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Bacteriophage infection is targeted to cellular poles.Molecular microbiology · 2008Article
- The Min system as a general cell geometry detection mechanism: branch lengths in Y-shaped Escherichia coli cells affect Min oscillation patterns and division dynamics.Journal of bacteriology · 2008Article
- Bacterial growth and cell division: a mycobacterial perspective.Microbiology and molecular biology reviews : MMBR · 2008Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Certain mutants in Escherichia coli lacking multiple penicillin-binding proteins (PBPs) produce misshapen cells containing kinks, bends and branches. These deformed regions exhibit two structural characteristics of normal cell poles: the peptidoglycan is inert to dilution by new synthesis or turnover, and a similarly stable patch of outer membrane caps the sites. To test the premise that these aberrant sites represent biochemically functional but misplaced cell poles, we assessed the intracellular distribution of proteins that localize specifically to bacterial poles. Green fluorescent protein (GFP) hybrids containing polar localization sequences from the Shigella flexneri IcsA protein or from the Vibrio cholerae EpsM protein formed foci at the poles of wild-type E. coli and at the poles and morphological abnormalities in PBP mutants. In addition, secreted wild-type IcsA localized to the outer membrane overlying these aberrant domains. We conclude that the morphologically deformed sites in these mutants represent fully functional poles or pole fragments. The results suggest that prokaryotic morphology is driven, at least in part, by the controlled placement of polar material, and that one or more of the low-molecular-weight PBPs participate in this process. Such mutants may help to unravel how particular proteins are targeted to bacterial poles, thereby creating important biochemical and functional asymmetries.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.