ArticleMarine biotechnology (New York, N.Y.)
Zebrafish cDNA encoding multifunctional Fatty Acid elongase involved in production of eicosapentaenoic (20:5n-3) and docosahexaenoic (22:6n-3) acids.
Article in Marine biotechnology (New York, N.Y.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 141 citations in OpenAlex.
- Molecular Identification and Functional Characterization of LC-PUFA Biosynthesis Elongase (Animals : an open access journal from MDPI · 2024Article
- Contribution ofAnimals : an open access journal from MDPI · 2024Article
- Genome-wide identification and expression profile of Elovl genes in threadfin fish Eleutheronema.Scientific reports · 2023Article
- Dominant Elongase Activity ofInternational journal of molecular sciences · 2022Article
- A chromosome-level genome assembly of the jade perch (Scortum barcoo).Scientific data · 2022Article
- Article
- Association between the Polymorphisms ofAnimals : an open access journal from MDPI · 2021Article
- Lack of ∆5 Desaturase Activity Impairs EPA and DHA Synthesis in Fish Cells from Red Sea Bream and Japanese Flounder.Marine biotechnology (New York, N.Y.) · 2021Article
- Article
- The requirements for sterol regulatory element-binding protein (Srebp) and stimulatory protein 1 (Sp1)-binding elements in the transcriptional activation of two freshwater fish Channa striata and Danio rerio elovl5 elongase.Fish physiology and biochemistry · 2020Article
- Molecular and Functional Characterization ofInternational journal of molecular sciences · 2020Article
- Functional diversification of teleost Fads2 fatty acyl desaturases occurs independently of the trophic level.Scientific reports · 2019Article
- A Novel Dietary Source of EPA and DHA: Metabolic Engineering of an Important Freshwater Species-Common Carp by fat1-Transgenesis.Marine biotechnology (New York, N.Y.) · 2019Article
- Genomic, Transcriptomic, and Epigenomic Features Differentiate Genes That Are Relevant for Muscular Polyunsaturated Fatty Acids in the Common Carp.Frontiers in genetics · 2019Article
- Transcriptional activation of zebrafish fads2 promoter and its transient transgene expression in yolk syncytial layer of zebrafish embryos.Scientific reports · 2018Article
- Article
- Towards the development of a sustainable soya bean-based feedstock for aquaculture.Plant biotechnology journal · 2017Article
- Effects of dietary lipids on the hepatopancreas transcriptome of Chinese mitten crab (Eriocheir sinensis).PloS one · 2017Article
- Evolutionary functional elaboration of the Elovl2/5 gene family in chordates.Scientific reports · 2016Article
- Article
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5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Enzymes that increase the chain length of fatty acids are essential for biosynthesis of highly unsaturated fatty acids. The gLELO gene encodes a protein involved in the elongation of polyunsaturated fatty acids in the fungus Mortierella alpina. A search of the GenBank database identified several expressed sequence tag sequences, including one obtained from zebrafish (Danio rerio), with high similarity to gLELO. The full-length transcript ZfELO, encoding a polypeptide of 291 amino acid residues, was isolated from zebrafish liver cDNA. The predicted amino acid sequence of the open reading frame shared high similarity with the elongases of Caenorhabditis elegans and human. When expressed in Saccharomyces cerevisiae, the zebrafish open reading frame conferred the ability to lengthen the chain of a range of C18, C20, and C22 polyunsaturated fatty acids, indicating not only that biosynthesis of 22:6n-3 from 18:3n-3 via a 24-carbon intermediate is feasible, but also that one elongase enzyme can perform all three elongation steps required. The zebrafish enzyme was also able to elongate monounsaturated and saturated fatty acids, and thus demonstrates a greater level of promiscuity in terms of substrate use than any elongase enzyme described previously.
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