ArticleProceedings of the National Academy of Sciences of the United States of America2004
Reconstitution of retroviral fusion and uncoating in a cell-free system.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed.
- The avian retroviral receptor Tva mediates the uptake of transcobalamin bound vitamin B12 (cobalamin).Journal of virology · 2021Article
- Specific Interaction between eEF1A and HIV RT Is Critical for HIV-1 Reverse Transcription and a Potential Anti-HIV Target.PLoS pathogens · 2015Article
- Pinpointing retrovirus entry sites in cells expressing alternatively spliced receptor isoforms by single virus imaging.Retrovirology · 2014Article
- Visualization of the two-step fusion process of the retrovirus avian sarcoma/leukosis virus by cryo-electron tomography.Journal of virology · 2012Article
- Eukaryotic elongation factor 1 complex subunits are critical HIV-1 reverse transcription cofactors.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Synchronized retrovirus fusion in cells expressing alternative receptor isoforms releases the viral core into distinct sub-cellular compartments.PLoS pathogens · 2012Article
- Strand transfer and elongation of HIV-1 reverse transcription is facilitated by cell factors in vitro.PloS one · 2010Article
- Cell factors stimulate human immunodeficiency virus type 1 reverse transcription in vitro.Journal of virology · 2008Article
- Evidence for direct involvement of the capsid protein in HIV infection of nondividing cells.PLoS pathogens · 2007Article
- The road to chromatin - nuclear entry of retroviruses.Nature reviews. Microbiology · 2007Review
- Reverse transcription complex: the key player of the early phase of HIV replication.Future virology · 2007Article
- How TRIM5alpha defends against retroviral invasions.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Isolation of cell lines that show novel, murine leukemia virus-specific blocks to early steps of retroviral replication.Journal of virology · 2005Article
- ATPgammaS disrupts human immunodeficiency virus type 1 virion core integrity.Journal of virology · 2005Article
- Low pH is required for avian sarcoma and leukosis virus Env-dependent viral penetration into the cytosol and not for viral uncoating.Journal of virology · 2004Article
- Retroviruses 2004: review of the 2004 Cold Spring Harbor Retroviruses Conference.Retrovirology · 2004Article
- In defense of the cell: TRIM5alpha interception of mammalian retroviruses.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
The molecular events underlying the immediate steps of retroviral uncoating, occurring after membrane fusion and leading to the formation of an active reverse transcription complex, are not known. To better understand these processes, we have developed a cell-free system that recapitulates these early steps of retroviral replication by using avian sarcoma and leukosis virus as a model retrovirus. The substrates used in this system are viral particles that are trapped before completing membrane fusion. These virions are induced to fuse out of endosomes and the viral cores are released into solution where they are amenable to biochemical manipulation. This system revealed that membrane fusion is not sufficient to stimulate the formation of a reverse transcription complex. Instead, ATP hydrolysis and cellular factors >5 kDa in size are required. Furthermore, later steps of avian sarcoma and leukosis virus reverse transcription were stimulated by nuclear factors. The cell-free system should now allow for the definition of retroviral uncoating mechanisms and facilitate the identification and characterization of the cellular factors involved.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.