ArticleApplied and environmental microbiology2004
Multiplex PCR assay for detection of bacterial pathogens associated with warm-water Streptococcosis in fish.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 153 citations in OpenAlex.
- Review
- Article
- Serotype distribution and antibiogram ofMicrobiology spectrum · 2023Article
- Phenotypic and Genotypic Characterization of Antimicrobial Resistance in Streptococci Isolated from Human and Animal Clinical Specimens.Current microbiology · 2023Article
- Complete Genome Sequences of Streptococcus parauberis Serotype Ia, Ib/Ic, and II Isolates from Diseased Olive Flounder in South Korea.Microbiology resource announcements · 2023Article
- Prevalence of bacterial infections and the use of multiplex PCR assay for rapid detection of pathogens in cultured fish in Ghana.Archives of microbiology · 2022Article
- Robust Nanozyme-Enzyme Nanosheets-Based Lactate Biosensor for Diagnosing Bacterial Infection in Olive Flounder (Biosensors · 2021Article
- First Identification and Characterization ofAnimals : an open access journal from MDPI · 2020Article
- Serotypes, virulence genes and polymorphism of capsule gene cluster inIranian journal of veterinary research · 2020Article
- Immunogenicity of a bivalent protein as a vaccine against Edwardsiella anguillarum and Vibrio vulnificus in Japanese eel (Anguilla japonica).MicrobiologyOpen · 2019Article
- Efficacy and safety data of ceftiofur antibiotics againstData in brief · 2019Article
- Gene expression profiles alteration after infection of virus, bacteria, and parasite in the Olive flounder (Paralichthys olivaceus).Scientific reports · 2018Article
- Complete Genome Sequence of Multiple-Antibiotic-Resistant Streptococcus parauberis Strain SPOF3K, Isolated from Diseased Olive Flounder (Paralichthys olivaceus).Genome announcements · 2018Article
- Transcriptome and Proteome of Fish-PathogenicFrontiers in microbiology · 2018Article
- Genetic and pathogenic difference between Streptococcus agalactiae serotype Ia fish and human isolates.BMC microbiology · 2016Article
- Analysis of zebrafish (Danio rerio) behavior in response to bacterial infection using a self-organizing map.BMC veterinary research · 2015Article
- Development of a multiplex PCR assay to detect Edwardsiella tarda, Streptococcus parauberis, and Streptococcus iniae in olive flounder (Paralichthys olivaceus).Journal of veterinary science · 2014Article
- Combining molecular evolution and environmental genomics to unravel adaptive processes of MHC class IIB diversity in European minnows (Phoxinus phoxinus).Ecology and evolution · 2013Article
- Development of a Reverse Line Blot Hybridization method for Detection of some Streptococcal/Lactococcal Species, the causative agents of Zoonotic Streptococosis/Lactococosis in farmed fish.Iranian journal of microbiology · 2012Article
- Complete genome sequence and immunoproteomic analyses of the bacterial fish pathogen Streptococcus parauberis.Journal of bacteriology · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A multiplex PCR-based method was designed for the simultaneous detection of the main pathogens involved in warm-water streptococcosis in fish (Streptococcus iniae, Streptococcus difficilis, Streptococcus parauberis, and Lactococcus garvieae). Each of the four pairs of oligonucleotide primers exclusively amplified the targeted gene of the specific microorganism. The sensitivity of the multiplex PCR using purified DNA was 25 pg for S. iniae, 12.5 pg for S. difficilis, 50 pg for S. parauberis, and 30 pg for L. garvieae. The multiplex PCR assay was useful for the specific detection of the four species of bacteria not only in pure culture but also in inoculated fish tissue homogenates and naturally infected fish. Therefore, this method could be a useful alternative to the culture-based method for the routine diagnosis of warm-water streptococcal infections in fish.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.