ArticleApplied and environmental microbiology2004
Two distinct pathways for trehalose assimilation in the yeast Saccharomyces cerevisiae.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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42 citing papers in PubMed, 121 citations in OpenAlex.
- A Possible Involvement of Sialidase in the Cell Response of the Antarctic FungusLife (Basel, Switzerland) · 2025Article
- Key Amino Acid Residues of the Agt1 Transporter for Trehalose Transport byJournal of fungi (Basel, Switzerland) · 2024Article
- Evaluating cellular roles and phenotypes associated with trehalose degradation genes in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2024Article
- Optimization of Citrus Pulp Waste-Based Medium for Improved Bacterial Nanocellulose Production.Microorganisms · 2024Article
- Enhancing freeze-thaw tolerance in baker's yeast: strategies and perspectives.Food science and biotechnology · 2024Review
- How do the carbon and nitrogen sources affect the synthesis of β-(1,3/1,6)-glucan, its structure and the susceptibility of Candida utilis yeast cells to immunolabelling with β-(1,3)-glucan monoclonal antibodies?Microbial cell factories · 2024Article
- Improved Sugarcane-Based Fermentation Processes by an Industrial Fuel-Ethanol Yeast Strain.Journal of fungi (Basel, Switzerland) · 2023Article
- Article
- Deletion of NTH1 and HSP12 increases the freeze-thaw resistance of baker's yeast in bread dough.Microbial cell factories · 2022Article
- Whole-cell modeling in yeast predicts compartment-specific proteome constraints that drive metabolic strategies.Nature communications · 2022Article
- Kinetic Modeling ofMetabolites · 2022Review
- Kveik Brewing Yeasts Demonstrate Wide Flexibility in Beer Fermentation Temperature Tolerance and Exhibit Enhanced Trehalose Accumulation.Frontiers in microbiology · 2022Article
- The involvement of theVirulence · 2021Article
- Article
- The secreted acid trehalase encoded by the CgATH1 gene is involved in Candida glabrata virulence.Memorias do Instituto Oswaldo Cruz · 2020Article
- The Inhibitory Effect of Validamycin A onInternational journal of microbiology · 2020Article
- Review
- Article
- Discovery and Functional Characterization of a Yeast Sugar Alcohol Phosphatase.ACS chemical biology · 2018Article
- Trehalose-6-phosphate promotes fermentation and glucose repression inMicrobial cell (Graz, Austria) · 2018Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The yeast Saccharomyces cerevisiae can synthesize trehalose and also use this disaccharide as a carbon source for growth. However, the molecular mechanism by which extracellular trehalose can be transported to the vacuole and degraded by the acid trehalase Ath1p is not clear. By using an adaptation of the assay of invertase on whole cells with NaF, we showed that more than 90% of the activity of Ath1p is extracellular, splitting of the disaccharide into glucose. We also found that Agt1p-mediated trehalose transport and the hydrolysis of the disaccharide by the cytosolic neutral trehalase Nth1p are coupled and represent a second, independent pathway, although there are several constraints on this alternative route. First, the AGT1/MAL11 gene is controlled by the MAL system, and Agt1p was active in neither non-maltose-fermenting nor maltose-inducible strains. Second, Agt1p rapidly lost activity during growth on trehalose, by a mechanism similar to the sugar-induced inactivation of the maltose permease. Finally, both pathways are highly pH sensitive and effective growth on trehalose occurred only when the medium was buffered at around pH 5.0. The catabolism of trehalose was purely oxidative, and since levels of Ath1p limit the glucose flux in the cells, batch cultures on trehalose may provide a useful alternative to glucose-limited chemostat cultures for investigation of metabolic responses in yeast.
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