ArticleApplied and environmental microbiology2004
Characterization of humanized antibodies secreted by Aspergillus niger.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 107 citations in OpenAlex.
- The landscape of antibody production systems: recombinant expression for research, diagnostics and therapy.Frontiers in bioengineering and biotechnology · 2026Review
- Section-level genome sequencing and comparative genomics ofStudies in mycology · 2025Article
- Expression of microbial lipase in filamentous fungus3 Biotech · 2024Review
- Review
- Stepwise genetic modification for efficient expression of heterologous proteins in Aspergillus nidulans.Applied microbiology and biotechnology · 2023Article
- Vesicular Stomatitis Virus (VSV) G Glycoprotein Can Be Modified to Create a Her2/Neu-Targeted VSV That Eliminates Large Implanted Mammary Tumors.Journal of virology · 2023Article
- Development of an auto-inducible expression system by nitrogen sources switching based on the nitrogen catabolite repression regulation.Microbial cell factories · 2022Article
- Challenges of influencing cellular morphology by morphology engineering techniques and mechanical induced stress on filamentous pellet systems-A critical review.Engineering in life sciences · 2021Review
- Improved Homologous Expression of the Acidic Lipase fromJournal of microbiology and biotechnology · 2020Article
- Functional production of human antibody by the filamentous fungusFungal biology and biotechnology · 2020Article
- Glycoengineering of Antibodies for Modulating Functions.Annual review of biochemistry · 2019Review
- Challenging the workhorse: Comparative analysis of eukaryotic micro-organisms for expressing monoclonal antibodies.Biotechnology and bioengineering · 2019Article
- A Review of the Microbial Production of Bioactive Natural Products and Biologics.Frontiers in microbiology · 2019Review
- Extracellular Expression in Aspergillus niger of an Antibody Fused to Leishmania sp. Antigens.Current microbiology · 2018Article
- Expression of naturally ionic liquid-tolerant thermophilic cellulases in Aspergillus niger.PloS one · 2017Article
- Enabling low cost biopharmaceuticals: high level interferon alpha-2b production in Trichoderma reesei.Microbial cell factories · 2016Article
- Making recombinant proteins in filamentous fungi- are we expecting too much?Frontiers in microbiology · 2014Review
- Human extracellular superoxide dismutase (EC-SOD) expression in transgenic chicken.BMB reports · 2013Article
- Expression of recombinant antibodies.Frontiers in immunology · 2013Article
- Ligation Independent Cloning of Polycistronic, Genetically Modified, HuMAb4D5-8 F (ab') 2, in Bacterial Plasmid.Avicenna journal of medical biotechnology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two different humanized immunoglobulin G1(kappa) antibodies and an Fab' fragment were produced by Aspergillus niger. The antibodies were secreted into the culture supernatant. Both light and heavy chains were initially synthesized as fusion proteins with native glucoamylase. After antibody assembly, cleavage by A. niger KexB protease allowed the release of free antibody. Purification by hydrophobic charge induction chromatography proved effective at removing any antibody to which glucoamylase remained attached. Glycosylation at N297 in the Fc region of the heavy chain was observed, but this site was unoccupied on approximately 50% of the heavy chains. The glycan was of the high-mannose type, with some galactose present, and the size ranged from Hex(6)GlcNAc(2) to Hex(15)GlcNAc(2). An aglycosyl mutant form of antibody was also produced. No significant difference between the glycosylated antibody produced by Aspergillus and that produced by mammalian cell cultures was observed in tests for affinity, avidity, pharmacokinetics, or antibody-dependent cellular cytotoxicity function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.