ArticleThe Biochemical journal2004
TIRF imaging of docking and fusion of single insulin granule motion in primary rat pancreatic beta-cells: different behaviour of granule motion between normal and Goto-Kakizaki diabetic rat beta-cells.
Article in The Biochemical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
72 citing papers in PubMed, 172 citations in OpenAlex.
- ForePass outperforms Semaglutide in weight control, glucose metabolism, and gut microbiota in swine.Diabetes, obesity & metabolism · 2025Article
- Multiple pathways and independent functional pools in insulin granule exocytosis.Genes to cells : devoted to molecular & cellular mechanisms · 2023Review
- SNAP23 decreases insulin secretion by competitively inhibiting the interaction between SNAP25 and STX1A.Bioscience reports · 2023Article
- Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis.eLife · 2023Article
- Munc13b stimulus-dependently accumulates on granuphilin-mediated, docked granules prior to fusion.Cell structure and function · 2022Article
- Auto-segmentation and time-dependent systematic analysis of mesoscale cellular structure in β-cells during insulin secretion.PloS one · 2022Article
- β-Cell Pathophysiology: A Review of Advanced Optical Microscopy Applications.International journal of molecular sciences · 2021Review
- In vivo Roles of Rab27 and Its Effectors in Exocytosis.Cell structure and function · 2021Review
- Article
- A fluorescent timer reporter enables sorting of insulin secretory granules by age.The Journal of biological chemistry · 2020Article
- Article
- A basic protein, N25, from a mollusk modifies calcium carbonate morphology and shell biomineralization.The Journal of biological chemistry · 2019Article
- Insulin secretory granules labelled with phogrin-fluorescent proteins show alterations in size, mobility and responsiveness to glucose stimulation in living β-cells.Scientific reports · 2019Article
- In situ graphene liquid cell-transmission electron microscopy study of insulin secretion in pancreatic islet cells.International journal of nanomedicine · 2019Article
- Monitoring real-time hormone release kinetics via high-content 3-D imaging of compensatory endocytosis.Lab on a chip · 2018Article
- MiR-335 overexpression impairs insulin secretion through defective priming of insulin vesicles.Physiological reports · 2017Article
- A Ratiometric Sensor for Imaging Insulin Secretion in Single β Cells.Cell chemical biology · 2017Article
- Article
- Opposing roles for SNAP23 in secretion in exocrine and endocrine pancreatic cells.The Journal of cell biology · 2016Article
- Granuphilin exclusively mediates functional granule docking to the plasma membrane.Scientific reports · 2016Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We imaged and analysed the motion of single insulin secretory granules near the plasma membrane in live pancreatic beta-cells, from normal and diabetic Goto-Kakizaki (GK) rats, using total internal reflection fluorescence microscopy (TIRFM). In normal rat primary beta-cells, the granules that were fusing during the first phase originate from previously docked granules, and those during the second phase originate from 'newcomers'. In diabetic GK rat beta-cells, the number of fusion events from previously docked granules were markedly reduced, and, in contrast, the fusion from newcomers was still preserved. The dynamic change in the number of docked insulin granules showed that, in GK rat beta-cells, the total number of docked insulin granules was markedly decreased to 35% of the initial number after glucose stimulation. Immunohistochemistry with anti-insulin antibody observed by TIRFM showed that GK rat beta-cells had a marked decline of endogenous insulin granules docked to the plasma membrane. Thus our results indicate that the decreased number of docked insulin granules accounts for the impaired insulin release during the first phase of insulin release in diabetic GK rat beta-cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.