Evidence map›Paper›PMID 15126489›Full record

ArticleJournal of bacteriology2004

Genome-wide analysis of lipoprotein expression in Escherichia coli MG1655.

Stephen J Brokx, Michael Ellison, Troy Locke, Drell Bottorff, Laura Frost, Joel H Weiner

Abstract read
In one paragraph

Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Article
  6. The double life of a bacterial lipoprotein.Molecular microbiology · 2011
    Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Insights into the biology of Escherichia coli through structural proteomics.Journal of structural and functional genomics · 2007
    Review
  13. Article
  14. Article
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Stephen J BrokxProject CyberCell, Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada.
Michael Ellison
Troy Locke
Drell Bottorff
Laura Frost
Joel H Weiner

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

To gain insight into the cell envelope of Escherichia coli grown under aerobic and anaerobic conditions, lipoproteins were examined by using functional genomics. The mRNA expression levels of each of these genes under three growth conditions--aerobic, anaerobic, and anaerobic with nitrate--were examined by using both Affymetrix GeneChip E. coli antisense genome arrays and real-time PCR (RT-PCR). Many genes showed significant changes in expression level. The RT-PCR results were in very good agreement with the microarray data. The results of this study represent the first insights into the possible roles of unknown lipoprotein genes and broaden our understanding of the composition of the cell envelope under different environmental conditions. Additionally, these data serve as a test set for the refinement of high-throughput bioinformatic and global gene expression methods.

Indexed as

Genome, BacterialEscherichia coliLipoproteinsOligonucleotide Array Sequence AnalysisReverse Transcriptase Polymerase Chain ReactionLipoproteins

Identifiers

PMID15126489
PMCPMC400601

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.